Phenol Extraction of Heavy Rapidly-Labeled Nuclear RNA from Chick Embryos
| Autor: | Bozenna Olszanska, Zofia Lassota, Ewa Grabczewska |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Hot Temperature
Time Factors Fraction (chemistry) Chick Embryo Nucleic Acid Denaturation Tritium Biochemistry chemistry.chemical_compound Phenols Animals Phenol Polyacrylamide gel electrophoresis Cell Nucleus Chromatography Extraction (chemistry) Sodium Dodecyl Sulfate RNA Phenol extraction Hydrogen-Ion Concentration Ribosomal RNA Molecular Weight Electrophoresis Solubility chemistry RNA Ribosomal Isotope Labeling Electrophoresis Polyacrylamide Gel |
| Zdroj: | European Journal of Biochemistry. 42:367-376 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1974.tb03348.x |
| Popis: | 1 Heavy rapidly-labeled RNA from chick embryo nuclei separates on electrophoresis in 2.4% polyacrylamide gel into at least four fractions with the mobilities of rRNA precursors, and a fraction of “giant” RNA remaining at the start. 2 The heavy fractions with mobilities of rRNA precursors pass into solution without heating during phenol treatment at pH 8.3 or during phenol-1.6% dodecylsulphate treatment at pH 5. 3 The “giant” RNA can be partially solubilized without heating by phenol-1.6% dodecylsulphate treatment at pH 5 but the extraction becomes efficient only on heating to 65°C or on raising the pH to 8.3. 4 Precautions are discussed to prevent the degradation of heavy RNA at elevated pH and the aggregation of rRNA at elevated temperature during phenol extraction. |
| Databáze: | OpenAIRE |
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