Large neutral amino acids levels in primate cerebrospinal fluid do not confirm competitive transport under baseline conditions
| Autor: | Frances McClellan, Rodolfo Bongiovanni, George E. Jaskiw, John D. Elsworth, Ali S. Mchaourab, Manda Double |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Phenylalanine Biology Blood–brain barrier 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Interstitial fluid Internal medicine Chlorocebus aethiops medicine Animals Humans Tyrosine Molecular Biology Aged chemistry.chemical_classification General Neuroscience Tryptophan Biological Transport Transporter Middle Aged Amino acid Amino Acids Neutral 030104 developmental biology medicine.anatomical_structure Endocrinology chemistry Blood-Brain Barrier Neurology (clinical) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research. 1648:372-379 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2016.08.011 |
| Popis: | In rodents, transport of large neutral amino acids (LNAAs) across the blood brain barrier (BBB) and blood-cerebrospinal fluid (CSF) barrier is mediated by high affinity carriers. Net brain LNAA levels are thought to be determined mainly by this competitive transport from plasma. Since the affinity for LNAA transport at the BBB in primates is considerably higher than in rodents, brain influx and by extension LNAA brain levels, should be even more dependent on competitive transport. Given that LNAA levels in CSF and brain interstitial fluid are usually similar, we analyzed serum and CSF of fasted subjects (n=24) undergoing spinal anesthesia and calculated brain influx and transporter occupancy using a conventional model of transport. Despite predicted near-full transporter saturation (99.7%), correlations between CSF levels and brain influx were modest, limited to tyrosine (r=0.60, p |
| Databáze: | OpenAIRE |
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