Use of a non-extraction HPLC technique for measuring angiotensin-converting enzyme under optimum conditions
| Autor: | Michael Norman Badminton, T.R. Tickner, Sandra J. Rainbow, C M Dawson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Chromatography medicine.diagnostic_test Chemistry Clinical Biochemistry Kinetics Extraction (chemistry) Substrate (chemistry) General Medicine Peptidyl-Dipeptidase A High-performance liquid chromatography Sensitivity and Specificity Enzyme Chlorides Spectrophotometry Protein purification medicine Spectrophotometry Ultraviolet Quantitative analysis (chemistry) Oligopeptides Chromatography High Pressure Liquid |
| Zdroj: | Annals of clinical biochemistry. 28 |
| ISSN: | 0004-5632 |
| Popis: | A non-extraction high performance liquid chromatography assisted method for the measurement of angiotensin-converting enzyme in serum has been developed. Serum containing the enzyme was incubated with the synthetic substrate, furylacryloylphenylalanylglycylglycine at a concentration approaching 10 times the apparent Km, prior to injection onto a shielded hydrophobic reverse phase high performance liquid chromatography column. This allowed the product to be separated and measured directly without the need for a time consuming protein extraction step. The method is enzymatically sound and avoids the methodological problems associated with automated kinetic assays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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