Saikosaponin a Ameliorates LPS-Induced Acute Lung Injury in Mice

Autor: Zhan-Sheng Hu, Zhi-An Du, Mei-Na Sun
Rok vydání: 2017
Předmět:
Lipopolysaccharides
Male
0301 basic medicine
Lipopolysaccharide
Acute Lung Injury
Interleukin-1beta
Immunology
Anti-Inflammatory Agents
Pulmonary Edema
Lung injury
Pharmacology
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
stomatognathic system
NLR Family
Pyrin Domain-Containing 3 Protein
medicine
Animals
Immunology and Allergy
Oleanolic Acid
Lung
Mice
Inbred BALB C
Dose-Response Relationship
Drug
biology
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Caspase 1
NF-kappa B
Inflammasome
Saponins
respiratory system
respiratory tract diseases
Disease Models
Animal
stomatognathic diseases
030104 developmental biology
medicine.anatomical_structure
Bronchoalveolar lavage
Neutrophil Infiltration
chemistry
Myeloperoxidase
biology.protein
Nasal administration
Inflammation Mediators
Bronchoalveolar Lavage Fluid
medicine.drug
Zdroj: Inflammation. 41:193-198
ISSN: 1573-2576
0360-3997
DOI: 10.1007/s10753-017-0677-3
Popis: The purpose of this study was to investigate the protective effects of Saikosaponin a (SSa), a triterpene saponin derived from Radix bupleuri, on lipopolysaccharide (LPS)-induced acute lung injury (ALI) using a murine model. The mice were given SSa 1 h after intranasal instillation of LPS. Then, lung histopathological examination, the wet/dry (W/D) ratio, myeloperoxidase (MPO), and inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were detected in this study. The results showed that SSa reduced lung pathological injury induced by LPS. Furthermore, LPS-induced lung W/D ratio, MPO activity, and inflammatory cytokines TNF-α and IL-1β in BALF were significantly inhibited by SSa. In addition, SSa suppressed LPS-induced NF-κB activation and NLRP3 inflammasome expression. In conclusion, we found that SSa played a critical anti-inflammatory effect through inhibition of NF-κB and NLRP3 signaling pathways and protected against LPS-induced ALI.
Databáze: OpenAIRE
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