Bio-activity guided fractions, isolation and antimicrobial evaluation of active constituents of Plumbago zeylanica Linn

Autor: Sudhakara Reddy M, Sivasankar R, Udaykiran V, Muniya Naik M, Subahan M
Jazyk: angličtina
Rok vydání: 2022
Předmět:
DOI: 10.5281/zenodo.7364671
Popis: Plumbago zeylanica belonging to the family plumbaginaceae , proved to be a richer source of components with possible pharmacological values. Isolated from the root of this plant crude extracts were exhibited antimicrobial, antidermatophyte property, the active extracts were subjected for isolation of active constituent by using different techniques. On the basis of antimicrobial activity P. zeylanica have been chosen for the present study. The Petroleum ether, chloroform, ethanol and distilled water extracts of roots of P. zeylanica were prepared and these crude extracts were screened for their antimicrobial activity by agar dilution method. All the crude extracts has shown promising antimicrobial activity and the results were recorded in minimum inhibition concentration (MIC) values. The crude cold methanol extract showed inhibition at a concentration of 35 mg/ml for S. aureus. The results of antimicrobial activity are recorded as minimum inhibition concentration (MIC) value in µg/ml at the concentration where the growth of the microorganism is inhibited. Thus the plant Plumbago zeylanica proved to be active among the other plants chosen under bioactivity selection of plant. Root is considered more potent than others. Key words: Plumbago zeylanica L, Antimicrobial activity, Minimum Inhibitory Concentration (MIC), Crude extracts. REFERENCES Farnsworth, N.R & Bringel, A.S (1986). Problems and prospects of discovering new drugs from higher plants by pharmacological screening. Wagner, H. and Wolff P. ed., New natural products and plant drugs with pharmacological biological or therapeutic activity, Springer, New York, 1-22. Farnsworth, N.R (1998). Screening of plants for New medicines, Wilson ed. Biodiversity National Academy Press, Washington DC, Part II, 83-97. Narayana, D.B.A., C.K. Katayarand N.B. Brindavanam, (1998). Original system: search, research or re-search.IDMA Bulletin, 29: 413-416. Mukherjee, P.K., Manoranjan, S & Suresh, B (1998). Indian herbal medicinesThe Eastern Pharmacist,42, 21-24. Anonymous (1959). Wealth of India-Raw Materials, CSIR, New Delhi. Kirtikar, K.R & Basu, B.D (1987). Indian Medicinal Plants, International Book Distributors, Dehradun. Chopra, R.N., Nayar, S.L & Chopra, I.C (1999). Glossary of Indian Medicinal Plants, 5th edition, National Institute of Science Communications. New Delhi Behl, P.N., Arora, R.B., Srivastava, G & Malhotra, S.C (1993). Herbs: useful in dermatological therapy, 1st edition, S.K. Jain for CBS Publishers and Distributors, pp. 66-67. Anonymous (1948). Wealth of India- Raw Materials, CSIR, New Delhi. Kirtikar, K.R & Basu, B.D (2000). Indian Medical Plants, Sri Satguru Publications, New Delhi, 3rd Edition, Vol. 3, p. 873. Kirtikar, K.R & Basu, B.D (2000). Indian Medicinal Plants, Sri Satguru Publications, New Delhi, Vol. 6, pp. 2030-2031. Kirtikar, K.R & Basu, B.D (2000). Indian Medicinal Plants, Sri Satguru Publications, New Delhi, 3rd edition, pp. 2205-2207. Behl, P.N., Arora, R.B., Srivastva, G & Malhotra, S.C (1993). Herbs: Useful in Dermatological therapy, 1st edition, pp. 15-116. Lin LC, Chou CJ. (2003). Meroterpenes and C-glucosylflavonoids from the aerial parts of Plumbago zeylanica. Chinese Pharm J; 55:77-81. Ahmed, I., Mehmood, Z & Mohammad, F (1998). Screening of some Indian medicinal plants for their antimicrobial properties. J. Ethnopharmacol., 62, ;62(2):183-93. Kini, D.P., Pandey, S., Shenoy, B.D., Singh, U.V & Udupa, N,Umadevi P.Kamath R (1997). Antitumar, and antifertility activities of plumbagin controlled release formulations. Indian J. Exp. Biol., 35, 374-379. Lin LC, Yang LL, Chou CJ. (2003). Cytotoxic naphthoquinones and plumbagic acid glucosides from Plumbago zeylanica. Phytochemistry; 62:619–622. Krishnaswamy, M & Purushattaman, K.K (1980). Indian J. Exp. Biol., 18, 876-877. Mukharya, D. and Dahia, M.S. (1977). Antimicrobial activity of some plant extract. Indian Drug, 14: 160-162. Jeevan Ram, A., Bhakshu, L & Venkata Raju, R.R (2004). In vitro antimicrobial activity of certain medicinal plants from eastern Ghats, India, used for skin diseases. J. Ethnopharmacol., 90,(2-3)353-357. Shen, Z., Doung, Z., Cheng, P., Chen, Z & Liu, J (2003). Effects of plumbagin on platelet aggregation and platelet-neutrophil interactions Planta Medica, 69, 605-609. Simonsen HT, Nordskjold JB, Smith UW, Nyman U, Palpu P, Joshi P et al. (2001) ; In vitro Screening of Indian medicinal plants for antiplasmodial activity. Journal of Ethnopharmacology 74:195-204. Veluri, R., Diwan, P. V. (1999). Phytochemical and pharmacological aspects of Plumbago zeylanica. Indian Drugs. 36(12): 724-730. Hazra, B., Sarkar, R., Bhattacharyya, S., Ghosh, P.K., Chel, G & Dinda, B (2002). Phytotherapy Res., 16, 133-137. Dinda, B., Hajra, A.K & Chel, G (1997). Naphthoquinones of Plumbago species J. Indian Chem. Soc., 74, 974-979. Abdul KM and Ramchander RP.( 1995). Modulatory effect of plumbagin (5-hydrozy-2-methyl -1,4-naphthoqunone) on macrophages function in BALB/c mice potentiation of macrophages bacterial activity. Immunopharmacology; 30 (3):231-236. Heble, M.R., Narayanaswamy, S & Chadha, M.S (1974). Tissue differentiation and plumbagin synthesis in variant cell strains of plumbagozeylanica L. in vitro. Plant Sci. Let., 6, 405-409. Olagunju JA, Jobi AA, Oyedapo OO. (1999). An investigation into the biochemical basis of the observed hyperglycemia in rats treated with ethanol root extract of Plumbago zeylanica Phytotherapy Research; 13(4):346-48. Mahishi, P., Srinivasa, B.H & Shivanna, M.B (2005).Medicinal plant wealth of local communities in some villages in shimoga District of Karnataka,India J. Ethnopharmacol., 98, 307-312. Gupta MM, Verma RK, Uniyal GC, Jain SP. (1993) Determination of plumbagin by normal phase high-performance liquid chromatography. J Chrom 637, 209–212 Sateesh Pujari, & Estari Mamidala. (2015). Anti-diabetic activity of Physagulin-F isolated from Physalis angulata fruits. The American Journal of Science and Medical Research, 1(2), 53–60. https://doi.org/10.5281/zenodo.7352308 Sharma I, Gusain D, Dixit VP. (1991). Hypolipidaemic and antiatherosclerotic effects of plumbagin in rabbits. Indian Journal of Physiology and Pharmacology; 35:10–14 Singh, U.V., Bisht, K.S., Rao, S., Uma Devi, P & Udupa, N (1997). Reduced toxicity and enhanced antitumor efficacy of plumbagin using poly (lactic-co-glycolic) biodegradable injectable implant Indian J. Pharmacol., 29, 168-172. Masataka, I., Kazumi, T & Hiroshi, F (1991) Cardiotonic action of plumbagin on guinea pig papillary muscle.. Planta Medica, 57, 317-319. Mehmood Z, Ahmad I, Mohammad F, Ahmad S. (1999). Indian medicinal plants: A potential source of anticandidal drugs. Pharmaceutical Biology; 37:237–242. Richa Tyagi, Ekta Menghani . Suresh Gyan .(2014) A Review on Plumabgo zeylanica : A Compelling Herb. International Journal of Pharma Sciences and Research (IJPSR). Vol 5 No 04. Richa Tyagi, Ekta Menghani, Suresh Gyan Vihar, (2014). Phytochemical screening of Plumbago zeylanica: A potent Herb International Journal of Pharma Sciences and Research (IJPSR) Vol 5 No 03
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