Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA
| Autor: | Hanno Sjuts, Yasmina Noubia Abdiche, Jennifer Clark, Stuart Knowling |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Computer science Binding properties Antibodies Monoclonal Biosensing Techniques Surface Plasmon Resonance Biochemistry Receptor–ligand kinetics Analytical Chemistry Automation Kinetics 03 medical and health sciences 0302 clinical medicine 030220 oncology & carcinogenesis Humans Molecular Medicine Biochemical engineering Surface plasmon resonance Protein Binding 030304 developmental biology Biotechnology Label free biosensor |
| Zdroj: | SLAS Discovery. 25:977-984 |
| ISSN: | 2472-5552 |
| Popis: | Funding pressure on the pharmaceutical industry to deliver new medicines to the market under aggressive timelines has led to a demand for analytical tools with higher detection sensitivity, increased throughput, and automation to speed up research and discovery efforts and converge upon clinically fit leads faster. In the quest for therapeutic antibodies, the early adoption of interaction analysis platforms utilizing surface plasmon resonance (SPR) detection provides insightful molecular-level information about the binding properties of antibody libraries that are key to understanding an antibody's mechanism of action and can guide the library-to-leads triage. Here, we sought to compare the binding kinetics obtained on two state-of-the-art high-throughput SPR platforms in an independent study conducted by unrelated groups located on different continents. We show that when experiments were performed by skilled users adhering to SPR best practices and allowed freedom in their assay design, the two platforms yielded near-identical results, establishing them both as reliable tools in accelerating the characterization of antibody libraries in providing critical information needed to advance leads to the clinic. |
| Databáze: | OpenAIRE |
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