Ionic currents in PC12 cells differentiated into neuron-like cells by a cultured-sciatic nerve conditioned medium
| Autor: | Cecilia Castillo, Gloria M. Villegas, Fanny Carreño, Raimundo Villegas |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Potassium Channels
Ionic bonding PC12 Cells Ion Channels Sodium Channels Membrane Potentials Nifedipine Neurotrophic factors Nerve Growth Factor Conditioned medium medicine Animals Nerve Growth Factors Molecular Biology Neurons Chemistry General Neuroscience Cell Differentiation Calcium Channel Blockers Sciatic Nerve In vitro Rats medicine.anatomical_structure nervous system Cell culture Culture Media Conditioned Biophysics Neurology (clinical) Neuron Sciatic nerve Calcium Channels Neuroscience Developmental Biology medicine.drug |
| Zdroj: | Brain research. 911(2) |
| ISSN: | 0006-8993 |
| Popis: | The present work deals with the identification of the ionic currents found in PC12 cells differentiated into neuron-like cells by a 9-11-day cultured-sciatic nerve conditioned medium (CM). PC12 whole-cell currents were measured after chronic exposure to CM. The results obtained in these CM-treated cells reveal that the functional expression of Ca(2+) currents is increased, that Na+ currents are not affected, and that a transient K+ current and a K+ delayed rectifier (K+ dr) current are increased. The combination of nifedipine and omega-conotoxin GVIA (omega-CgTX) does not block completely the increased functional expression of the Ca(2+) current. The remaining current is blocked by omega-agatoxin TK indicating that P/Q-type channels are additionally contributing to the increase in Ca(2+) current. NGF-treated PC12 cells, used as positive controls, confirm that NGF increases the expression of voltage-dependent Na+ currents and of Ca(2+) currents. In addition, we found that NGF also increases a K+ dr-type current in these cells. The results obtained with the CM might be due to a molecule or a mixture of molecules released into the medium by the 9-11-day cultured sciatic nerves. |
| Databáze: | OpenAIRE |
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