Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
| Autor: | Amps, K., Andrews, P.W., Anyfantis, G., Armstrong, L., Avery, S., Baharvand, H., Baker, J., Baker, D., Munoz, M.B., Beil, S., Benvenisty, N., Ben-Yosef, D., Biancotti, J.C., Bosman, A., Brena, R.M., Brison, D., Caisander, G., Camarasa, M.V., Chen, J.M., Chiao, E., Choi, Y.M., Choo, A.B.H., Collins, D., Colman, A., Crook, J.M., Daley, G.Q., Dalton, A., Sousa, P.A. de, Denning, C., Downie, J., Dvorak, P., Montgomery, K.D., Feki, A., Ford, A., Fox, V., Fraga, A.M., Frumkin, T., Ge, L., Gokhale, P.J., Golan-Lev, T., Gourabi, H., Gropp, M., Lu, G.X., Hampl, A., Harron, K., Healy, L., Herath, W., Holm, F., Hovatta, O., Hyllner, J., Inamdar, M.S., Irwanto, A.K., Ishii, T., Jaconi, M., Jin, Y., Kimber, S., Kiselev, S., Knowles, B.B., Kopper, O., Kukharenko, V., Kuliev, A., Lagarkova, M.A., Laird, P.W., Lako, M., Laslett, A.L., Lavon, N., Lee, D.R., Lee, J.E., Li, C.L., Lim, L.S., Ludwig, T.E., Ma, Y., Maltby, E., Mateizel, I., Mayshar, Y., Mileikovsky, M., Minger, S.L., Miyazaki, T., Moon, S.Y., Moore, H., Mummery, C., Nagy, A., Nakatsuji, N., Narwani, K., Oh, S.K.W., Oh, S.K., Olson, C., Otonkoski, T., Pan, F., Park, I.H., Pells, S., Pera, M.F., Pereira, L.V., Qi, O., Raj, G.S., Reubinoff, B., Robins, A., Robson, P., Rossant, J., Salekdeh, G.H., Schulz, T.C., Sermon, K., Mohamed, J.S., Shen, H., Sherrer, E., Sidhu, K., Sivarajah, S., Skottman, H., Spits, C., Stacey, G.N., Strehl, R., Strelchenko, N., Suemori, H., Sun, B.W., Suuronen, R., Takahashi, K., Tuuri, T., Venu, P., Verlinsky, Y., Ward-van Oostwaard, D., Weisenberger, D.J., Wu, Y., Yamanaka, S., Young, L., Zhou, Q., Int Stem Cell Initiative |
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| Přispěvatelé: | Bosman, Alexis, Feki, Anis, Jaconi, Marisa |
| Rok vydání: | 2011 |
| Předmět: |
Inhibitor of Differentiation Protein 1
Cellular differentiation Ethnic Groups/genetics Chromosomes Human Pair 20 Inhibitor of Differentiation Protein 1/genetics/metabolism Growth ddc:616.07 Applied Microbiology and Biotechnology 0302 clinical medicine Induced Pluripotent Stem Cells/cytology Ethnicity Growth/genetics Induced pluripotent stem cell Genetics CÉLULAS-TRONCO 0303 health sciences Gene Expression Regulation Developmental RNA-Binding Proteins Cell Differentiation Amplicon Molecular Medicine Stem cell Biotechnology Homeobox protein NANOG Genotype Cell Differentiation/genetics Induced Pluripotent Stem Cells bcl-X Protein Biomedical Engineering Bioengineering Biology Polymorphism Single Nucleotide Selection Genetic/genetics Cell Line Clonal Evolution 03 medical and health sciences Bcl-X Protein/genetics/metabolism Humans RNA-Binding Proteins/genetics/metabolism Selection Genetic Embryonic Stem Cells 030304 developmental biology Embryonic Stem Cells/cytology Genetic Variation DNA Methylation Molecular biology Embryonic stem cell Chromosomes Human Pair 20/genetics Clonal Evolution/genetics Cell culture Chromosome 20 030217 neurology & neurosurgery |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Nature Biotechnology, 29(12), 1132-U113 Nature Biotechnology, Vol. 29, No 12 (2011) pp. 1132-44 Amps, K, Andrews, P W, Anyfantis, G, Armstrong, L, Avery, S, Baharvand, H, Baker, J, Baker, D, Munoz, M B, Beil, S, Benvenisty, N, Ben-Yosef, D, Biancotti, J-C, Bosman, A, Brena, R M, Brison, D, Caisander, G, Camarasa, M V, Chen, J, Chiao, E, Choi, Y M, Choo, A B H, Collins, D, Colman, A, Crook, J M, Daley, G Q, Dalton, A, De Sousa, P A, Denning, C, Downie, J, Dvorak, P, Montgomery, K D, Feki, A, Ford, A, Fox, V, Fraga, A M, Frumkin, T, Ge, L, Gokhale, P J, Golan-Lev, T, Gourabi, H, Gropp, M, Lu, G, Hampl, A, Harron, K, Healy, L, Herath, W, Holm, F & Hovatta, O & Pells, S 2011, ' Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage ', Nature Biotechnology, vol. 29, no. 12, pp. 1132-1144 . https://doi.org/10.1038/nbt.2051 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt.2051 |
| Popis: | The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells. |
| Databáze: | OpenAIRE |
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