Immunotoxicity and genotoxicity testing of PLGA-PEO nanoparticles in human blood cell model
Autor: | Fedor Čiampor, Mira Horvathova, Alena Kazimirova, Dagmar Bilanicova, Eva Neubauerova, Aurelia Liskova, Marta Staruchova, Magdalena Barancokova, Martina Drlickova, Katarina Volkovova, Michal Cagalinec, Giulio Pojana, Miroslava Kuricova, Maria Dusinska, Eva Jahnova, Ivo Vávra, Maria Bartusova, Jana Tulinska |
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Rok vydání: | 2013 |
Předmět: |
Genotoxicity
Immunotoxicity Lymphocytes Natural killer cells Phagocytes and respiratory burst PLGA-PEO nanoparticles Cell Proliferation Cells Cultured Humans Lactic Acid Leukocytes Mononuclear Mutagenicity Tests Nanoparticles Phagocytosis Polyglycolic Acid Polylactic Acid-Polyglycolic Acid Copolymer Biomedical Engineering Toxicology Cells Mononuclear 02 engineering and technology Biology medicine.disease_cause Peripheral blood mononuclear cell 03 medical and health sciences chemistry.chemical_compound Antigen Leukocytes medicine Cytotoxicity Settore CHIM/12 - Chimica dell'Ambiente e dei Beni Culturali 030304 developmental biology Whole blood 0303 health sciences Cultured Cell growth technology industry and agriculture 021001 nanoscience & nanotechnology Molecular biology In vitro 3. Good health Lactic acid chemistry Immunology 0210 nano-technology |
Zdroj: | Nanotoxicology. 9:33-43 |
ISSN: | 1743-5404 1743-5390 |
Popis: | A human blood cell model for immunotoxicity and genotoxicity testing was used to measure the response to polylactic-co-glycolic acid (PLGA-PEO) nanoparticle (NP) (0.12, 3, 15 and 75 μg/cm(2) exposure in fresh peripheral whole blood cultures/isolated peripheral blood mononuclear cell cultures from human volunteers (n = 9-13). PLGA-PEO NPs were not toxic up to dose 3 μg/cm(2); dose of 75 μg/cm(2) displays significant decrease in [(3)H]-thymidine incorporation into DNA of proliferating cells after 4 h (70% of control) and 48 h (84%) exposure to NPs. In non-cytotoxic concentrations, in vitro assessment of the immunotoxic effects displayed moderate but significant suppression of proliferative activity of T-lymphocytes and T-dependent B-cell response in cultures stimulated with PWMCON A, and no changes in PHA cultures. Decrease in proliferative function was the most significant in T-cells stimulated with CD3 antigen (up to 84%). Cytotoxicity of natural killer cells was suppressed moderately (92%) but significantly in middle-dosed cultures (4 h exposure). On the other hand, in low PLGA-PEO NPs dosed cultures, significant stimulation of phagocytic activity of granulocytes (119%)monocytes (117%) and respiratory burst of phagocytes (122%) was recorded. Genotoxicity assessment revealed no increase in the number of micronucleated binucleated cells and no induction of SBs or oxidised DNA bases in PLGA-PEO-treated cells. To conclude on immuno- and genotoxicity of PLGA-PEO NPs, more experiments with various particle size, charge and composition need to be done. |
Databáze: | OpenAIRE |
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