Immunotoxicity and genotoxicity testing of PLGA-PEO nanoparticles in human blood cell model

Autor: Fedor Čiampor, Mira Horvathova, Alena Kazimirova, Dagmar Bilanicova, Eva Neubauerova, Aurelia Liskova, Marta Staruchova, Magdalena Barancokova, Martina Drlickova, Katarina Volkovova, Michal Cagalinec, Giulio Pojana, Miroslava Kuricova, Maria Dusinska, Eva Jahnova, Ivo Vávra, Maria Bartusova, Jana Tulinska
Rok vydání: 2013
Předmět:
Genotoxicity
Immunotoxicity
Lymphocytes
Natural killer cells
Phagocytes and respiratory burst
PLGA-PEO nanoparticles
Cell Proliferation
Cells
Cultured
Humans
Lactic Acid
Leukocytes
Mononuclear
Mutagenicity Tests
Nanoparticles
Phagocytosis
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Biomedical Engineering
Toxicology
Cells
Mononuclear
02 engineering and technology
Biology
medicine.disease_cause
Peripheral blood mononuclear cell
03 medical and health sciences
chemistry.chemical_compound
Antigen
Leukocytes
medicine
Cytotoxicity
Settore CHIM/12 - Chimica dell'Ambiente e dei Beni Culturali
030304 developmental biology
Whole blood
0303 health sciences
Cultured
Cell growth
technology
industry
and agriculture
021001 nanoscience & nanotechnology
Molecular biology
In vitro
3. Good health
Lactic acid
chemistry
Immunology
0210 nano-technology
Zdroj: Nanotoxicology. 9:33-43
ISSN: 1743-5404
1743-5390
Popis: A human blood cell model for immunotoxicity and genotoxicity testing was used to measure the response to polylactic-co-glycolic acid (PLGA-PEO) nanoparticle (NP) (0.12, 3, 15 and 75 μg/cm(2) exposure in fresh peripheral whole blood cultures/isolated peripheral blood mononuclear cell cultures from human volunteers (n = 9-13). PLGA-PEO NPs were not toxic up to dose 3 μg/cm(2); dose of 75 μg/cm(2) displays significant decrease in [(3)H]-thymidine incorporation into DNA of proliferating cells after 4 h (70% of control) and 48 h (84%) exposure to NPs. In non-cytotoxic concentrations, in vitro assessment of the immunotoxic effects displayed moderate but significant suppression of proliferative activity of T-lymphocytes and T-dependent B-cell response in cultures stimulated with PWMCON A, and no changes in PHA cultures. Decrease in proliferative function was the most significant in T-cells stimulated with CD3 antigen (up to 84%). Cytotoxicity of natural killer cells was suppressed moderately (92%) but significantly in middle-dosed cultures (4 h exposure). On the other hand, in low PLGA-PEO NPs dosed cultures, significant stimulation of phagocytic activity of granulocytes (119%)monocytes (117%) and respiratory burst of phagocytes (122%) was recorded. Genotoxicity assessment revealed no increase in the number of micronucleated binucleated cells and no induction of SBs or oxidised DNA bases in PLGA-PEO-treated cells. To conclude on immuno- and genotoxicity of PLGA-PEO NPs, more experiments with various particle size, charge and composition need to be done.
Databáze: OpenAIRE
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