Molecular characterization of Haemaphysalis longicornis-borne rickettsiae, Republic of Korea and China
| Autor: | Heung-Chul Kim, Ju Jiang, Sung-Tae Chong, Monica L. O’Guinn, Allen L. Richards, Roxanne G. Burrus, John S. Lee, Hui-juan An, Terry A. Klein, Yan-min Zhang, Yu-zhou Bao, Dan Song |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Nymph 0301 basic medicine China Ixodidae 030231 tropical medicine Zoology Tick Microbiology 03 medical and health sciences 0302 clinical medicine Rickettsiaceae Phylogenetics Rickettsia heilongjiangensis Republic of Korea Animals Phylogeny biology Phylogenetic tree Sequence Analysis DNA biology.organism_classification 030104 developmental biology Infectious Diseases Rickettsia Genes Bacterial Larva Insect Science Candidatus Multilocus sequence typing Female Parasitology Haemaphysalis longicornis |
| Zdroj: | Ticks and Tick-borne Diseases. 9:1606-1613 |
| ISSN: | 1877-959X |
| DOI: | 10.1016/j.ttbdis.2018.07.013 |
| Popis: | Haemaphysalis longicornis, the cattle tick or bush tick, has an extended distribution throughout Asia and the Pacific region, including China, Russia, the Republic of Korea (ROK), Japan, Australia, New Zealand, and the South Pacific islands. It is an obligate ectoparasite found commonly on medium to large sized wild and domestic animals, with humans as an accidental host. Haemaphysalis longicornis transmits a number of pathogens, including severe fever with thrombocytopenia syndrome and tick-borne encephalitis viruses, bacteria, helminths, and protozoans, that impact on veterinary (wild and domestic animals) and human health. Surveys of rickettsial pathogens associated with H. longicornis from China, the ROK, and Japan have resulted in the discovery of more than 35 incompletely characterized molecular isolates of Rickettsia. In response to the increased global threat of tick-borne rickettsial diseases, H. longicornis collected in the ROK and China were assessed in our laboratory and two additional Rickettsia spp. isolates (ROK-HL727 and XinXian HL9) were identified. These agents were fully characterized by multilocus sequence typing using partial gene fragment sequences of rrs, gltA, ompA, ompB, and sca4. Phylogenetic comparisons of these Rickettsia isolates with known Rickettsia species and other molecular isolates identified from H. longicornis were performed to better understand their interrelationships. Phylogenetic analysis of the sequences from these 5 gene fragments showed that ROK-HL727 was closely related to rickettsial isolates of H. longicornis previously reported from China, the ROK and Japan, but distinct from any currently recognized Rickettsia species. It therefore qualifies genetically as a new species, introduced herein as Candidatus Rickettsia longicornii. The XinXian-HL9 isolate detected from China was determined to be genetically similar to the human pathogen Rickettsia heilongjiangensis. People living and working in areas where H. longicornis is endemic should be aware of the potential for rickettsial diseases. |
| Databáze: | OpenAIRE |
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