Patterning factors during neural progenitor induction determine regional identity and differentiation potential in vitro
| Autor: | Dwayne Holmes, Lisa Gasparotto, Paulien Cornelissen-Steijger, Vivi M. Heine, Prisca S. Leferink, Aishwarya G. Nadadhur, Lisa Hinz |
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| Přispěvatelé: | Pediatric surgery, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, Amsterdam Neuroscience - Complex Trait Genetics, Amsterdam Neuroscience - Compulsivity, Impulsivity & Attention, Amsterdam Neuroscience - Mood, Anxiety, Psychosis, Stress & Sleep, Complex Trait Genetics |
| Rok vydání: | 2018 |
| Předmět: |
Pluripotent Stem Cells
0301 basic medicine Cell type Retinoic acid Patterning factors Biology Cell Line 03 medical and health sciences chemistry.chemical_compound In vitro Pluripotent stem cells Neural Stem Cells medicine Humans Progenitor cell Induced pluripotent stem cell lcsh:QH301-705.5 Neural cell Homeodomain Proteins Neurons Lineage markers Neural tube Cell Differentiation Cell Biology General Medicine Neural progenitors Cell biology 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) chemistry Astrocytes Neural development Transcription Factors Developmental Biology |
| Zdroj: | Stem Cell Research, 32, 25-34. Elsevier Nadadhur, A G, Leferink, P S, Holmes, D, Hinz, L, Cornelissen-Steijger, P, Gasparotto, L & Heine, V M 2018, ' Patterning factors during neural progenitor induction determine regional identity and differentiation potential in vitro ', Stem Cell Research, vol. 32, pp. 25-34 . https://doi.org/10.1016/j.scr.2018.08.017 Stem Cell Research, Vol 32, Iss, Pp 25-34 (2018) |
| ISSN: | 1873-5061 |
| Popis: | The neural tube consists of neural progenitors (NPs) that acquire different characteristics during gestation due to patterning factors. However, the influence of such patterning factors on human pluripotent stem cells (hPSCs) during in vitro neural differentiation is often unclear. This study compared neural induction protocols involving in vitro patterning with single SMAD inhibition (SSI), retinoic acid (RA) administration and dual SMAD inhibition (DSI). While the derived NP cells expressed known NP markers, they differed in their NP expression profile and differentiation potential. Cortical neuronal cells generated from 1) SSI NPs exhibited less mature neuronal phenotypes, 2) RA NPs exhibited an increased GABAergic phenotype, and 3) DSI NPs exhibited greater expression of glutamatergic lineage markers. Further, although all NPs generated astrocytes, astrocytes derived from the RA-induced NPs had the highest GFAP expression. Differences between NP populations included differential expression of regional identity markers HOXB4, LBX1, OTX1 and GSX2, which persisted into mature neural cell stages. This study suggests that patterning factors regulate how potential NPs may differentiate into specific neuronal and glial cell types in vitro. This challenges the utility of generic neural induction procedures, while highlighting the importance of carefully selecting specific NP protocols. Keywords: Neural progenitors, Patterning factors, In vitro, Pluripotent stem cells, Astrocytes, Neurons |
| Databáze: | OpenAIRE |
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