miR375-3p Distinguishes Low-Grade Neuroendocrine From Non-neuroendocrine Lung Tumors in FFPE Samples
| Autor: | Simone Detassis, Valerio del Vescovo, Margherita Grasso, Stefania Masella, Chiara Cantaloni, Luca Cima, Alberto Cavazza, Paolo Graziano, Giulio Rossi, Mattia Barbareschi, Leonardo Ricci, Michela Alessandra Denti |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Context (language use) Neuroendocrine tumors Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry miR-375 03 medical and health sciences 0302 clinical medicine microRNA Medicine neuroendocrine Molecular Biosciences Lung cancer Molecular Biology lcsh:QH301-705.5 Cause of death Lung business.industry respiratory system Brief Research Report medicine.disease Paraffin embedded lung cancer 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) 030220 oncology & carcinogenesis Biomarker (medicine) biomarker business |
| Zdroj: | Frontiers in Molecular Biosciences Frontiers in Molecular Biosciences, Vol 7 (2020) |
| ISSN: | 2296-889X |
| Popis: | Lung cancer is still one of the leading cause of death worldwide. The clinical variability of lung cancer is high and drives treatment decision. In this context, correct discrimination of pulmonary neuroendocrine tumors is still of critical relevance. The spectrum of neuroendocrine tumors is various, and each type has molecular and phenotypical differences. In order to advance in the discrimination of neuroendocrine from non-neuroendocrine lung tumors, we tested a series of 95 surgically resected and formalin-fixed paraffin embedded lung cancer tissues, and we analyzed the expression of miR205-5p and miR375-3p via TaqMan RT-qPCR. Via a robust mathematical approach, we excluded technical outliers increasing the data reproducibility. We found that miR375-3p levels are higher in low-grade neuroendocrine lung tumor samples compared to non-neuroendocrine lung tumors. However, miR375-3p is not able to distinguish among different types of neuroendocrine lung tumors. In this work, we provide a new molecular marker for distinguishing non-neuroendocrine from low-grade neuroendocrine lung tumors samples establishing an easy miRNA score to be used in clinical settings, enabling the pathologist to classify more accurately lung tumors biopsies, which may be ambiguously cataloged in routine examination. |
| Databáze: | OpenAIRE |
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