A plasma membrane-associated phospholipase in SV40-transformed 3T3 cells
| Autor: | Herbert Voelz, Dominic Fan |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Cell
Phosphatidylserines Simian virus 40 Phospholipase Biology Phospholipases A 3T3 cells Cell Line Substrate Specificity Mice chemistry.chemical_compound medicine Animals chemistry.chemical_classification Cell Membrane Substrate (chemistry) Serum Albumin Bovine Cell Biology Phosphatidylserine Cell Transformation Viral Membrane medicine.anatomical_structure Enzyme chemistry Biochemistry Phospholipases Cytochemistry Calcium |
| Zdroj: | Experimental Cell Research. 126:47-55 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(80)90469-3 |
| Popis: | Cells of a SV3T3 line can be adapted to degrade phosphatides made into a film. A phosphatide degrading enzyme(s) was localized at cell surface structures by cytochemistry. Biochemistry of cell fractions indicated the presence of a phospholipase which was bound to the plasma membrane. It has a substrate specificity for phosphatidylserine, a pH optimum in the acid region, and is inhibited by calcium. It is not dissociated from the membrane by products of phospholipid degradation and is presumably a phospholipase A. The hydrolysis of the substrate under experimental conditions is about 32%. The cell biological importance of the enzyme is discussed in regard to cellular interactions and invasiveness of virus-transformed cells. |
| Databáze: | OpenAIRE |
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