Microchamber microfluidics combined with thermogellable glycomicrogels - Platform for single cells study in an artificial cellular microenvironment
| Autor: | Sebastian Student, Ziemowit Ostrowski, Malgorzata Milewska, K. Gut, Ilona Wandzik |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Microfluidics Population Cell Culture Techniques Bioengineering Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Time-lapse microscopy Biomaterials Exponential growth Microsystem Cellular Microenvironment Humans education Cell Proliferation education.field_of_study 021001 nanoscience & nanotechnology 0104 chemical sciences Mechanics of Materials Cell culture Biomimetic Devices 0210 nano-technology HeLa Cells |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 119 |
| ISSN: | 1873-0191 |
| Popis: | Microfluidic technology is a powerful tool to precisely establish artificial microenvironments and has been used to generate numerous biomimetic devices. Here, we present a combined microenvironment platform, which consists of microchamber microfluidics filled with thermoresponsive glycomicrogels, and enables live-cell immobilization and continuous observation. Poly(N-isopropylacrylamide) microgels containing trehalose has been selected from our previous study to possess adequate physicochemical characteristics and provide potential multivalent interactions with cell surfaces. We show that the designed microplatform enables small population of cells to be trapped in individual parallel microchambers and further immobilized in an artificial extracellular matrix. We applied our platform to long-term imaging experiments and studied HeLa cell growth dynamics under continuous, diffusion-dominated medium exchange. The mathematical modeling revealed that regardless of the initial number of cells, the growth dynamic follows the exponential growth pattern over the analyzed timespan (one week). These results confirm that the presented microsystem facilitates the long-term cell culture in a cellular-mimicking microenvironment without reaching environmental constraints. |
| Databáze: | OpenAIRE |
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