| Autor: |
Piepke, Marius, Clausen, Bettina H., Ludewig, Peter, Vienhues, Jonas H., Bedke, Tanja, Javidi, Ehsan, Rissiek, Bj��rn, Jank, Larissa, Brockmann, Leonie, Sandrock, Inga, Degenhardt, Karoline, Jander, Alina, Roth, Vanessa, Sch��dlich, Ines S., Prinz, Immo, Flavell, Richard A., Kobayashi, Yasushi, Renn��, Thomas, Gerloff, Christian, Huber, Samuel, Magnus, Tim, Gelderblom, Mathias |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.17004434.v1 |
| Popis: |
Additional file 1: Figure S1. Il10���/��� mice exhibit worsened neurological scores compared to WT littermate controls, whereas mortality did not differ between groups. (A) Bederson score and survival rate; (B) 14 days after tMCAO in WT control and Il10���/��� mice. Figure S2. Generation of bone-marrow chimeric mice and characterization of post-ischemic IL-10 producers. (A) Generation of bone-marrow chimeras. (B) Representative FACS plots of peripheral blood of bone-marrow chimeras after sacrifice. (C) Flow cytometric analysis of IL-10 produced by B cells, NK cells, CD8+, and CD4+ T cells after sham surgery and 3, 7, and 14 days after stroke in FIR-tiger mice. (D) Expression of Tr1 cell markers CD49b and Lag-3 on CD4+ T cells in spleen and CNS 3 days after tMCAO in FIR-tiger mice. Figure S3. IL-10 deficiency leads to an activation of the IL-17A axis and IL-17A positive ���� T cellls co-express PD-1 in the post ischemic brain. (A) Flow cytometric analysis of the number of infiltrating IL-17A+ CD4+ and ���� T cells isolated from ischemic hemispheres of WT controls and Il10���/��� mice 7 days after tMCAO. (B) Relative gene expression of Cxcl1 and Mmp3 in ischemic hemispheres of WT and Il10���/��� mice 3 days after tMCAO. (C) Flow cytometric analysis of IL-17 production and PD-1 co-expression by ���� T cells in cervical lymph nodes and the CNS 3 days after stroke. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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