Activin A-induced increase in LOX activity in human granulosa-lutein cells is mediated by CTGF
Autor: | Christian Klausen, Jung-Chien Cheng, Peter C.K. Leung, Hsun-Ming Chang, Congjian Xu, Yingtao Liu |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
endocrine system Embryology Lysyl oxidase Gene Expression Regulation Enzymologic Extracellular matrix Protein-Lysine 6-Oxidase 03 medical and health sciences 0302 clinical medicine Endocrinology Downregulation and upregulation Ovarian Follicle Transforming Growth Factor beta Luteal Cells medicine Humans Ovarian follicle Granulosa Lutein Cell Activin type 2 receptors Cells Cultured Granulosa Cells integumentary system biology Chemistry Connective Tissue Growth Factor Obstetrics and Gynecology Cell Biology Transforming growth factor beta Cell biology Activins CTGF 030104 developmental biology medicine.anatomical_structure Reproductive Medicine 030220 oncology & carcinogenesis biology.protein Female |
Zdroj: | Reproduction (Cambridge, England). 152(4) |
ISSN: | 1741-7899 |
Popis: | Lysyl oxidase (LOX) is the key enzyme involved in the crosslinking of collagen and elastin that is essential for the formation of extracellular matrix (ECM). LOX-mediated ECM remodeling plays a critical role in follicle development, oocyte maturation and corpus luteum formation. To date, the regulation of LOX in human ovary has never been elucidated. Activin A and its functional receptors are highly expressed in ovarian follicles from an early developmental stage. They locally regulate follicle progression. The aim of this study was to investigate the effects of activin A on the expression of LOX and its extracellular enzyme activity in primary and immortalized human granulosa–lutein cells obtained from patients undergoing anin vitrofertilization procedure. We demonstrated that activin A significantly upregulated the expression of connective tissue growth factor (CTGF) and LOX via an activin/TGF-β type I receptor mediated-signaling pathway. Using a target depletion small interfering RNA knockdown approach, we further confirmed that the upregulation of CTGF expression resulted in an activin-A-induced increases in LOX expression and activity. These findings may provide insight into the mechanisms by which intrafollicular growth factors regulate the expression of LOX for ECM formation and tissue remodeling in the human ovary. |
Databáze: | OpenAIRE |
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