Cloning and expression of a pseudo-ovalbumin gene
| Autor: | Savio L. C. Woo, Bert W. O'Malley, Myles L. Mace, Ming-Jer Tsai, Eugene C. Lai, Sophia Y. Tsai |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
animal structures
Transcription Genetic Ovalbumin DNA Recombinant Biophysics Oviducts Nucleic Acid Denaturation Biochemistry Homology (biology) chemistry.chemical_compound Escherichia coli Animals RNA Messenger Molecular Biology Gene Messenger RNA biology Nucleic acid sequence Nucleic Acid Hybridization RNA Cell Biology respiratory system Molecular biology Kinetics Microscopy Electron Genes chemistry Protein Biosynthesis biology.protein Oviduct Female Chickens DNA Plasmids |
| Zdroj: | Biochemical and Biophysical Research Communications. 89:997-1005 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(79)91876-x |
| Popis: | A Pseudo-ovalbumin gene, bearing significant nucleotide sequence homology to the ovalbumin gene, has been cloned from genomic chick DNA. Similar to the authentic ovalbumin gene, the pseudo-gene is a unique sequence gene in the chick genome and is expressed at a low level in the immature chick oviduct. In contrast to the ovalbumin gene, expression of the pseudo-gene in the oviduct is not inducible by estrogen. The concentration of pseudo-gene RNA is only ∼0.01% of that of authentic ovalbumin mRNA in estrogen-stimulated oviduct cells. Nucleotide sequence analysis of the two sequence related genes may reveal the molecular basis of differential response to steroid hormone induction in the same tissue. |
| Databáze: | OpenAIRE |
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