Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis
| Autor: | Irina Makarova, L. E. Andreeva, V V Nenasheva, L. V. Gening, Vyacheslav Z. Tarantul, A. V. Lakhin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA polymerase DNA repair DNA polymerase II DNA polymerase delta Primer extension Article 03 medical and health sciences chemistry.chemical_compound lcsh:QH301-705.5 Molecular Biology 030102 biochemistry & molecular biology biology DNA synthesis DNA polymerase iota Cell Biology Molecular biology loach 030104 developmental biology lcsh:Biology (General) chemistry biology.protein DNA Polymerase Iota DNA Developmental Biology |
| Zdroj: | Journal of Developmental Biology Journal of Developmental Biology, Vol 4, Iss 1, p 6 (2016) Journal of Developmental Biology; Volume 4; Issue 1; Pages: 6 |
| ISSN: | 2221-3759 |
| Popis: | Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In transgenic loach overexpressing human Pol ι we have shown that the bypassing of DNA synthesis arrest after incorporation of mismatched nucleotide by Pol ι (the T-stop) was not associated with this enzyme. Non-transgenic loach larvae are virtually lacking the capacity for error correction of DNA duplex containing a mismatched nucleotide. Such repair activity develops only in the adult fish. It appears that the initial stages of development are characterized by more intensive DNA synthesis, while in terminal stages the repair activities become more prominent. The misGvA approach clearly indicates substantial changes in the DNA synthesis intensity, although the role of particular replicative and repair DNA polymerases in this process requires further study. |
| Databáze: | OpenAIRE |
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