Incidence of Mycobacterium avium subspecies paratuberculosis in a population-based cohort of patients with Crohn's disease and control subjects
| Autor: | Murray L. Barclay, Martin A. Kennedy, Richard B. Gearry, Jacqui Keenan, Robert W Bentley, Rebecca L. Roberts |
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| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial medicine.medical_specialty Genotype Population Nod2 Signaling Adaptor Protein Disease Polymerase Chain Reaction Sensitivity and Specificity Cohort Studies Crohn Disease Epidemiology Paratuberculosis Medicine Humans Mass Screening education education.field_of_study Crohn's disease Hepatology biology business.industry Incidence (epidemiology) Incidence Gastroenterology biology.organism_classification medicine.disease Control subjects Mycobacterium avium subspecies paratuberculosis digestive system diseases Mycobacterium avium subsp. paratuberculosis Cross-Sectional Studies Immunology Cohort business New Zealand |
| Zdroj: | The American journal of gastroenterology. 103(5) |
| ISSN: | 1572-0241 |
| Popis: | To define the incidence of Mycobacterium avium subspecies paratuberculosis (MAP) in patients with Crohn's disease (CD) and in control subjects.Blood samples from 361 CD patients from a previously described population-based inflammatory bowel disease (IBD) cohort and 200 blood donor controls, of known NOD2 genotype, were screened by PCR for MAP-specific IS900 DNA. These results were correlated with NOD2 genotype.The PCR assay was capable of detecting 20 fg of purified MAP DNA, equivalent to roughly 100 MAP cells/mL of blood. MAP-specific IS900 DNA was detected in 33.8% of CD cases and 21.5% of controls (OR 1.86, 95% CI 1.247-2.785, P= 0.002). All study participants were genotyped for the NOD2 mutations 2104CT (R702W), 2722GC (G908R), and 3020insC (1007fs). Carriage of one or two NOD2 mutations was not associated with a significantly higher risk of CD (OR 0.75, 95% CI 0.465-1.207, P= 0.234). No significant association was seen in the CD cohort for carriage of one or two NOD2 mutations and MAP status (OR 0.883, 95% CI 0.494-1.579, P= 0.675).Screening peripheral blood using IS900 PCR indicated that MAP DNA could be detected in a significant proportion of CD cases from a large population-based cohort, and also, in control subjects. The over-representation of MAP DNA in CD suggests either a role or a probable role for MAP in the etiology of CD. |
| Databáze: | OpenAIRE |
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