Pressurized liquid extraction of berberine and aristolochic acids in medicinal plants
| Autor: | Soo-On Woo, Eng Shi Ong, Yuk-Lin Yong |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Chromatography
Plants Medicinal biology Berberine Chemistry Alkaloid Organic Chemistry Extraction (chemistry) Reproducibility of Results Ranunculaceae General Medicine Pharmacognosy Phenanthrenes Reference Standards biology.organism_classification Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry chemistry.chemical_compound Aristolochiaceae Aristolochic Acids Medicinal plants Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. A. 904(1) |
| ISSN: | 0021-9673 |
| Popis: | Berberine and aristolochic acids I and II present naturally in medicinal plants were extracted using a laboratory-made pressurized liquid extraction (PLE) system in the dynamic mode. As the target analytes were present naturally in the medicinal plants, spiking was not done and comparison with ultrasonic extraction and Soxhlet extraction was performed to assess the method accuracy. The effect of temperature, volume of solvent required and particle size were investigated. Method precision (RSD, n=5) between 1.98 and 3.4% was achieved for the extraction of berberine and aristolochic acids I and II in medicinal plants and lower than 8% for lower levels of aristolochic acid II in medicinal plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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