Cytocompatibility and Cellular Internalization of PEGylated 'Clickable' Nucleic Acid Oligomers
| Autor: | Justine Wagner, Alex J. Anderson, Erica B. Peters, Stephanie J. Bryant, Christopher N. Bowman, Benjamin D. Fairbanks, Alex Neumann |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Polymers and Plastics media_common.quotation_subject Oligonucleotides Bioengineering 02 engineering and technology 3T3 cells Polyethylene Glycols Biomaterials Rhodamine Mice 03 medical and health sciences chemistry.chemical_compound PEG ratio Materials Chemistry medicine Animals Viability assay Internalization media_common Organelles Oligonucleotide Chemistry 3T3 Cells 021001 nanoscience & nanotechnology Endocytosis 030104 developmental biology medicine.anatomical_structure Biochemistry Drug delivery Nucleic acid 0210 nano-technology |
| Zdroj: | Biomacromolecules. 19:2535-2541 |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | The recently developed synthetic oligonucleotides referred to as "click" nucleic acids (CNAs) are promising due to their relatively simple synthesis based on thiol-X reactions with numerous potential applications in biotechnology, biodetection, gene silencing, and drug delivery. Here, the cytocompatibility and cellular uptake of rhodamine tagged, PEGylated CNA copolymers (PEG-CNA-RHO) were evaluated. NIH 3T3 fibroblast cells treated for 1 h with 1, 10, or 100 μg/mL PEG-CNA-RHO maintained an average cell viability of 86%, which was not significantly different from the untreated control. Cellular uptake of PEG-CNA-RHO was detected within 30 s, and the amount internalized increased over the course of 1 h. Moreover, these copolymers were internalized within cells to a higher degree than controls consisting of either rhodamine tagged PEG or the rhodamine alone. Uptake was not affected by temperature (i.e., 4 or 37 °C), suggesting a passive uptake mechanism. Subcellular colocalization analysis failed to indicate significant correlations between the internalized PEG-CNA-RHO and the organelles examined (mitochondria, endoplasmic reticulum, endosomes and lysosomes). These results indicate that CNA copolymers are cytocompatible and are readily internalized by cells, supporting the idea that CNAs are a promising alternative to DNA in antisense therapy applications. |
| Databáze: | OpenAIRE |
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