MicroRNA-21 protects from mesangial cell proliferation induced by diabetic nephropathy in db/db mice
| Autor: | Zheng Zhang, Xin Chen, Junxia Chen, Fei Han, Ning Yan, Xiaoming Xu, Xiaoyan He, Huimin Peng |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Biophysics Down-Regulation Diabetic nephropathy Transfection Biochemistry Mice Phosphatidylinositol 3-Kinases Downregulation and upregulation Structural Biology In vivo Diabetes mellitus Internal medicine microRNA Genetics medicine Animals PTEN Diabetic Nephropathies Molecular Biology Cells Cultured Cell Proliferation Base Sequence biology Mesangial cell business.industry Cell growth PTEN Phosphohydrolase Cell Biology medicine.disease Mice Mutant Strains Mice Inbred C57BL MicroRNAs Endocrinology Mesangial Cells biology.protein business Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | FEBS Letters. 583:2009-2014 |
| ISSN: | 0014-5793 |
| Popis: | Diabetic nephropathy (DN) is a major diabetic complication. But the initiating molecular events triggering DN are unknown. Recent researches have addressed the role of microRNAs in diabetes and its complications. In this study, we looked for microRNAs expression during early DN, and showed microRNA-21 (miR-21) expression was downregulated in response to early DN in vitro and in vivo. Over-expression of miR-21 inhibited proliferation of mesangial cells and decreased the 24-h urine albumin excretion rate in diabetic db/db mice. Moreover, we identified PTEN as a target of miR-21. We also found PI3K and p-Akt increased in miR-21 treated mesangial cells and db/db mice. Overall, these studies for the first time provide evidence for the potential role of miR-21 in early DN. |
| Databáze: | OpenAIRE |
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