Virulence and pathogenesis of yellow fever virus serially passaged in cell culture
| Autor: | Robert M. Kovatch, Ernest M. Snyder, James D. Pulliam, Stanley C. Nagle, John L. Converse |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Virus Cultivation
Swine Virulence Aedes aegypti Kidney General Biochemistry Genetics and Molecular Biology Virus Microbiology Cell Line Mice Serial passage Aedes Culture Techniques Yellow Fever medicine Animals General Pharmacology Toxicology and Pharmaceutics Infectivity General Immunology and Microbiology biology Yellow fever General Medicine Haplorhini medicine.disease biology.organism_classification Virology Culture Media Intestines Cell culture Autopsy Clinical Microbiology Virology and Immunology Yellow fever virus HeLa Cells |
| Zdroj: | Applied microbiology. 21(6) |
| ISSN: | 0003-6919 |
| Popis: | Viscerotropic virulence of the Asibi strain of yellow fever virus (YFV) for monkeys has been known to be lost after serial passage in HeLa cell monolayers. This phenomenon was investigated in several other mammalian and insect tissue cell lines. Assay in monkeys of original seed virus and of virus after 7 and 11 passages in a porcine kidney cell line (PK) indicated essentially equal infectivity and mortality. Moreover, monkeys receiving the passaged virus exhibited more rapid onset of disease and death than animals infected with original seed virus. Histological changes in animals inoculated with passaged virus were identical to those in animals receiving the seed virus. Virus from later passages in PK cells was also lethal for approximately 50% of the monkeys; however, evidence for progressive attenuation was seen in these preparations. Similar results were obtained with a mosquito ( Aedes aegypti ) cell line. In contrast to results obtained in PK and mosquito cells, YFV became essentially avirulent (nonlethal and less infective) for monkeys after only seven passages in HeLa cell cultures. |
| Databáze: | OpenAIRE |
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