MicroRNAs from the parasitic plantCuscuta campestristarget host messenger RNAs
| Autor: | Saima Shahid, Gunjune Kim, Nathan R. Johnson, Eric Wafula, Feng Wang, Ceyda Coruh, Vivian Bernal-Galeano, Tamia Phifer, Claude W. dePamphilis, James H. Westwood, Michael J. Axtell |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Parasitic plant MRNA cleavage food and beverages Nicotiana benthamiana Cuscuta campestris biology.organism_classification 01 natural sciences Cell biology 03 medical and health sciences Haustorium Gene silencing Arabidopsis thaliana Cuscuta 030304 developmental biology 010606 plant biology & botany |
| Popis: | First paragraphDodders (Cuscutaspp.) are obligate parasitic plants that obtain water and nutrients from the stems of host plants via specialized feeding structures called haustoria. Dodder haustoria facilitate bi-directional movement of viruses, proteins, and mRNAs between host and parasite1, but the functional effects of these movements are not clear. Here we show thatC. campestrishaustoria accumulate high levels of many novel microRNAs (miRNAs) while parasitizingArabidopsis thalianahosts. Many of these miRNAs are 22 nts long, a usually rare size of plant miRNA associated with amplification of target silencing through secondary small interfering RNA (siRNA) production2. SeveralA. thalianamRNAs are targeted byC. campestris22 nt miRNAs during parasitism, resulting in mRNA cleavage, secondary siRNA production, and decreased mRNA accumulation levels. Hosts with mutations in two of the targets supported significantly higher growth ofC. campestris.Homologs of target mRNAs from diverse plants also have predicted target sites to inducedC. campestrismiRNAs, and the same miRNAs are expressed and active against host targets whenC. campestrisparasitizes a different host,Nicotiana benthamiana. These data show thatC. campestrismiRNAs act astrans-species regulators of host gene expression, and suggest that they may act as virulence factors during parasitism. |
| Databáze: | OpenAIRE |
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