The pro-apoptosis effect of sinomenine in renal carcinoma via inducing autophagy through inactivating PI3K/AKT/mTOR pathway
| Autor: | Jing Feng, Ping Zhang, Yuexian Ma, Liang Liang, Fei Deng |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Apoptosis 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Autophagy Humans Mechanistic target of rapamycin Protein kinase B Carcinoma Renal Cell PI3K/AKT/mTOR pathway Sinomenine Cell Proliferation Pharmacology biology Cell growth Chemistry TOR Serine-Threonine Kinases RPTOR General Medicine Antineoplastic Agents Phytogenic Kidney Neoplasms 030104 developmental biology Morphinans 030220 oncology & carcinogenesis biology.protein Cancer research Signal transduction Phosphatidylinositol 3-Kinase Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 97 |
| ISSN: | 1950-6007 |
| Popis: | Renal cell carcinoma (RCC) is a heterogeneous histological disease and the most common kidney cancer. The mortality rate of RCC remains high despite the improved treatment. Sinomenine is an isoquinoline extracted from Chinese medicinal plant Sinomenium acutum, famous for its ability to suppress several cancer cell types. Our research aimed to explore the anti-cancer potential of sinomenine in RCC. Results showed that sinomenine reduced the viability by reducing sphere-forming ability and enhancing pro-apoptosis effect in ACHN cells in a dose dependent manner. The expression levels of proliferation/apoptosis markers further validated the result. In addition, sinomenine significantly regulated the level of autophagy-related proteins with decreased expression of p62, and increased Beclin1 and LC3 II/LC3 I. Furthermore, phosphatidylinositol-3 kinase (PI3K)/AKT/mechanistic target of rapamycin (mTOR), the negatively regulated cell autophagy signaling pathway, was inhibited by sinomenine with decreased membrane translocation of AKT in ACHN cell lines. All in all, our study demonstrated that sinomenine promoted apoptosis in RCC via enhancing autophagy through PI3K/AKT/mTOR pathway. |
| Databáze: | OpenAIRE |
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