Triazole-linked phenyl derivatives: Redox mechanisms and in situ electrochemical evaluation of interaction with dsDNA
| Autor: | Ana Maria Oliveira-Brett, Caterina Lombardo, A. Dora R. Pontinha, Stephen Neidle |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
In situ
dsDNA electrochemical biosensor Stereochemistry Biophysics Triazole Biosensing Techniques 010402 general chemistry Electrochemistry 01 natural sciences Redox 03 medical and health sciences chemistry.chemical_compound Glassy carbon electrode Physical and Theoretical Chemistry Voltammetry 030304 developmental biology 0303 health sciences Chemistry Substituted triazole-linked phenyl compounds DNA Electrochemical Techniques General Medicine Hydrogen-Ion Concentration Triazoles Combinatorial chemistry Carbon 0104 chemical sciences Solutions Acridines Differential pulse voltammetry Oxidation-Reduction Biosensor |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2014.08.010 |
| Popis: | The redox mechanism of two trisubstituted triazole-linked phenyl derivatives (CL41 and CL42) and a disubstituted triazole-linked phenyl derivative (CL2r50) were studied using cyclic, differential pulse and square wave voltammetry at a glassy carbon electrode. The CL41, CL42 and CL2r50 oxidation is a complex, pH-dependent irreversible process involving the formation of electroactive products that undergo two consecutive reversible oxidation reactions. The DNA interaction with CL41, CL42 and CL2r50 was investigated by differential pulse voltammetry using the dsDNA-electrochemical biosensor and in DNA/trisubstituted triazole incubated solutions. All three trisubstituted triazole-linked phenyl derivatives interacted with dsDNA causing morphological and oxidative damage to the dsDNA structure in a time-dependent manner. The DNA-electrochemical biosensor enabled the detection of oxidative damage to DNA following the occurrence of the 8-oxoGua and/or 2,8-oxoAde oxidation peaks. |
| Databáze: | OpenAIRE |
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