Methanolic extract of Ephedra ciliata promotes wound healing and arrests inflammatory cascade in vivo through downregulation of TNF-α
| Autor: | Maria Yaseen, Rizwan Rashid Bazmi, Hafiza Sidra Yaseen, Muhammad Usman Shams, Mohammad Saleem, Muhammad Asif, Malik Saadullah, Hafiz Muhammad Yousaf, Sajid Asghar, Mahrukh |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Staphylococcus aureus Ephedra Indomethacin Immunology Anti-Inflammatory Agents Down-Regulation Microbial Sensitivity Tests Pharmacology Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Anti-Infective Agents In vivo Candida albicans Animals Pharmacology (medical) Skin Inflammation Wound Healing biology Plant Extracts Tumor Necrosis Factor-alpha Ciliata Methanol Antimicrobial biology.organism_classification Rats Carrageenan Plant Leaves 030104 developmental biology chemistry Phytochemical Female Quercetin Aspergillus niger Wound healing 030217 neurology & neurosurgery |
| Zdroj: | Inflammopharmacology. 28:1691-1704 |
| ISSN: | 1568-5608 0925-4692 |
| Popis: | Chronic wounds may lead to the development of various pathological conditions such as diabetic foot ulcers and pressure sores. The current study evaluated wound healing and anti-inflammatory potentials of methanolic extract of Ephedra ciliata using series of in vivo models. Methanolic extract of Ephedra ciliata was prepared by maceration (Ec.Me). Qualitative and quantitative (HPLC) phytochemical and metal analyses were conducted to explore the chemical and metal profiles of Ec.Me. Safety profile (behavioural) and, antimicrobial, antioxidant, wound healing, anti-inflammatory and anti-angiogenic potentials of Ec.Me were evaluated using well-established in vitro and in vivo models. ELISA assay was performed to estimate the effects of Ec.Me treatment on serum levels of TNF-α. HPLC analysis identified quercetin as one of the major compounds in Ec.Me. Safety study data showed that Ec.Me was safe up to the dose of 2000 mg/kg. Antimicrobial assay data showed that Ec.Me was active against bacterial (Staphylococcus aureus) as well as fungal (Candida albicans and Aspergillus niger) strains. Ec.Me showed modertate antioxidant potential in in vitro and in vivo models. Data of excision and burn wound healing models showed that Ec.Me, promoted wound closure in a dose and time-dependent manner. Treatment with 20% Ec.Me cream and heparin showed almost the same effects with no statistical differences (p > 0.05). Ec.Me also showed time-dependent anti-inflammatory activities in both acute and chronic models. In carrageenan model, treatment with 200 mg/kg of Ec.Me showed comparable anti-inflammatory effects (p > 0.05) with quercetin and indomethacin throughout the study. In cotton pellet granuloma model treatment with 200 mg/kg of Ec.Me and indomethacin inhibited granuloma formation significantly better (p |
| Databáze: | OpenAIRE |
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