Association (micellization) and partitioning of aglycon triterpenoids
| Autor: | Marjan Rafat, T. G. Sambandan, Kit Wah Fong, Chokyun Rha, Brian C. Stephenson, Arthur Goldsipe, Samuel T. Coradetti, Anthony J. Sinskey |
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| Rok vydání: | 2008 |
| Předmět: |
Lung Neoplasms
Molecular Conformation Mass spectrometry Micelle Biomaterials Inhibitory Concentration 50 Colloid and Surface Chemistry Desorption Cell Line Tumor Humans Surface Tension Computer Simulation Solubility Carcinoma Small Cell Chromatography High Pressure Liquid Micelles Aggregation number Chromatography Chemistry Reversed-phase chromatography Triterpenes Surfaces Coatings and Films Electronic Optical and Magnetic Materials Partition coefficient Matrix-assisted laser desorption/ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Biological Assay Glioblastoma Pentacyclic Triterpenes |
| Zdroj: | Journal of colloid and interface science. 325(2) |
| ISSN: | 1095-7103 |
| Popis: | Micellization and solution properties of the aglycon triterpenoids asiatic acid (AA) and madecassic acid (MA) were examined experimentally and in computational simulations. AA and MA belong to the large class of bioactive aglycon triterpenoids, for which limited physicochemical data are available. In this study, solubility, partition coefficient, critical micelle concentrations (CMC), and surface tensions of AA and MA were measured. Reverse phase HPLC data, supported by dye probe experiments and drop shape analysis, showed the CMC in phosphate buffered saline (PBS) to be 15+/-2 microM, and 86+/-9 microM for AA and MA, respectively. The surface tensions of AA and MA in PBS were 64.1 and 64.4 mN/m, respectively. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry indicated the aggregation numbers of AA and MA to be 5 to 7. Molecular dynamics simulations confirmed that molecular association could occur between 5 and 7 molecules in solution. The IC(50) of AA and MA on human small cell carcinoma and human glioblastoma cell lines was 25+/-5 microM and 66+/-13 microM, respectively. The IC(50) is within the range of calculated CMC of AA and MA in bioassay media, suggesting that the micellar aggregates may lead to their cytotoxicity. |
| Databáze: | OpenAIRE |
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