Cytotoxic constituents of Abutilon indicum leaves against U87MG human glioblastoma cells

Autor: Rukaiyya Sirajuddin Khan, Mahibalan Senthi, Poorna Chandra Rao, Basha, Ameer, Alvala, Mallika, Tummuri, Dinesh, Masubuti, Hironori, Fujimoto, Yoshinori, Ahil Sajeli Begum
Rok vydání: 2015
DOI: 10.6084/m9.figshare.1251068.v1
Popis: The study was aimed to identify cytotoxic leads from Abutilon indicum leaves for treating glioblastoma. The petroleum ether extract, methanol extract (AIM), chloroform and ethyl acetate sub-fractions (AIM-C and AIM-E, respectively) prepared from AIM were tested for cytotoxicity on U87MG human glioblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. These extracts exhibited considerable activity (IC50 values of 42.6–64.5 μg/mL). The most active AIM-C fraction was repeatedly chromatographed to yield four known compounds, methyl trans-p-coumarate (1), methyl caffeate (2), syringic acid (3) and pinellic acid (4). Cell viability assay of 1–4 against U87MG cells indicated 2 as most active (IC50 value of 8.2 μg/mL), whereas the other three compounds were much less active. Interestingly, compounds 1–4 were non-toxic towards normal human cells (HEK-293). The content of 2 in AIM-C was estimated as 3% by HPLC. Hence, presence of some more active substances besides methyl caffeate (2) in AIM-C is anticipated.
Databáze: OpenAIRE