Natural Killer Cells from Malignant Pleural Effusion Are Endowed with a Decidual-Like Proangiogenic Polarization

Autor: Matteo Desio, D. G. D’Urso, Alessandra Musco, Lorenzo Dominioni, Silvia Zanellato, Annalisa Bosi, Douglas M. Noonan, Lorenzo Mortara, Elisa Nardecchia, Andrea Imperatori, Barbara Bassani, Maria Cattoni, Adriana Albini, Antonino Bruno
Přispěvatelé: Bosi, A, Zanellato, S, Bassani, B, Albini, A, Musco, A, Cattoni, M, Desio, M, Nardecchia, E, D'Urso, D, Imperatori, A, Dominioni, L, Noonan, D, Mortara, L, Bruno, A
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Cytotoxicity
Immunologic
Male
Malignant pleural effusion
Cellular differentiation
Cell Degranulation
0302 clinical medicine
Tumor Cell Polarization
Tumor Microenvironment
Immunology and Allergy
Cytotoxic T cell
Cells
Cultured
Aged
80 and over
Endothelial Cell
biology
Natural Killer cells
Malignant pleural effusion
Tumor Angiogenesis
Tumor Cell Polarization
Decidua
Degranulation
Cell Differentiation
General Medicine
Middle Aged
CD56 Antigen
Killer Cells
Natural
medicine.anatomical_structure
Tumor Angiogenesis
Female
Human
medicine.drug
Research Article
Interleukin 2
lcsh:Immunologic diseases. Allergy
Article Subject
Immunology
Neovascularization
Physiologic
03 medical and health sciences
Natural Killer cells
medicine
Humans
Aged
Tumor microenvironment
business.industry
Perforin
Receptors
IgG
Endothelial Cells
medicine.disease
Pleural Effusion
Malignant
030104 developmental biology
biology.protein
Cancer research
Interleukin-2
business
lcsh:RC581-607
030215 immunology
Zdroj: Journal of Immunology Research, Vol 2018 (2018)
Journal of Immunology Research
ISSN: 2314-7156
2314-8861
Popis: Natural killer (NK) cells are crucial in tumor recognition and eradication, but their activity is impaired in cancer patients, becoming poorly cytotoxic. A particular type of NK cells, from the decidua, has low cytotoxicity and shows proangiogenic functions. We investigated whether NK cells from peripheral blood (PB) and pleural effusions of patients develop decidual-like NK phenotype and whether exposure to IL-2 can restore their killing ability in the presence of pleural fluids. NK cells from pleural effusion of patients with inflammatory conditions (iPE, n = 18), primary tumor (ptPE, n = 18), and metastatic tumor (tmPE, n = 27) acquired the CD56brightCD16− phenotype. NK cells from both ptPE and tmPE showed increased expression for the CD49a and CD69 decidual-like (dNK) markers and decreased levels of the CD57 maturation marker. NK from all the PE analyzed showed impaired degranulation capability and reduced perforin release. PE-NK cells efficiently responded to IL-2 stimulation in vitro. Addition of TGFβ or cell-free pleural fluid to IL-2 in the culture medium abrogated NK cell CD107a and IFNγ expression even in healthy donors (n = 14) NK. We found that tmPE-NK cells produce VEGF and support the formation of capillary-like structures in endothelial cells. Our results suggest that the PE tumor microenvironment can shape NK cell polarization towards a low cytotoxic, decidual-like, highly proangiogenic phenotype and that IL-2 treatment is not sufficient to limit this process.
Databáze: OpenAIRE
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