Genomic Characteristics of Stx2e-Producing Escherichia coli Strains Derived from Humans, Animals, and Meats
Autor: | Bin Hu, Yanwen Xiong, Xiangning Bai, Hui Sun, Andreas Matussek, Xi Yang, Yannong Wu, Qian Liu, Ming Luo |
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Rok vydání: | 2021 |
Předmět: |
Microbiology (medical)
Serotype Tetracycline animal diseases Biology medicine.disease_cause Article Microbiology Shiga toxin Escherichia coli Stx2e whole genome sequencing fluids and secretions STX2 medicine Immunology and Allergy Molecular Biology Whole genome sequencing General Immunology and Microbiology Strain (biology) biochemical phenomena metabolism and nutrition bacterial infections and mycoses Multiple drug resistance Infectious Diseases biology.protein Medicine bacteria medicine.drug |
Zdroj: | Pathogens Pathogens, Vol 10, Iss 1551, p 1551 (2021) Pathogens; Volume 10; Issue 12; Pages: 1551 |
ISSN: | 2076-0817 |
Popis: | Shiga toxin (Stx) can be classified into two types, Stx1 and Stx2, and different subtypes. Stx2e is a subtype commonly causing porcine edema disease and rarely reported in humans. The purpose of this study was to analyze the prevalence and genetic characteristics of Stx2e-producing Escherichia coli (Stx2e-STEC) strains from humans compared to strains from animals and meats in China. Stx2e-STEC strains were screened from our STEC collection, and whole-genome sequencing was performed to characterize their genetic features. Our study showed a wide distribution of Stx2e-STEC among diverse hosts and a higher proportion of Stx2e-STEC among human STEC strains in China. Three human Stx2e-STEC isolates belonged to O100:H30, Onovel26:H30, and O8:H9 serotypes and varied in genetic features. Human Stx2e-STECs phylogenetically clustered with animal- and food-derived strains. Stx2e-STEC strains from animals and meat showed multidrug resistance, while human strains were only resistant to azithromycin and tetracycline. Of note, a high proportion (55.9%) of Stx2e-STEC strains, including one human strain, carried the heat-stable and heat-labile enterotoxin-encoding genes st and lt, exhibiting a STEC/enterotoxigenic E. coli (ETEC) hybrid pathotype. Given that no distinct genetic feature was found in Stx2e-STEC strains from different sources, animal- and food-derived strains may pose the risk of causing human disease. |
Databáze: | OpenAIRE |
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