Cyclic Arginine-Glycine-Aspartate Peptides Enhance Three-Dimensional Stem Cell Osteogenic Differentiation
| Autor: | Nathaniel Huebsch, Tanyarut Boontheekul, Susan X. Hsiong, David J. Mooney |
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| Rok vydání: | 2009 |
| Předmět: |
Cellular differentiation
Biomedical Engineering Bone Marrow Cells Bioengineering Stem cell factor Biology Peptides Cyclic Biochemistry Tissue Culture Techniques Biomaterials Mice Osteogenesis Animals Humans Bone regeneration Cell potency Cell Proliferation Induced stem cells Multipotent Stem Cells Stem Cells Cell Differentiation Cell biology Special Focus Papers Endothelial stem cell Immunology Stromal Cells Stem cell Adult stem cell |
| Zdroj: | Tissue Engineering Part A. 15:263-272 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2007.0411 |
| Popis: | The role of morphogens in bone regeneration has been widely studied, whereas the effect of matrix cues, particularly on stem cell differentiation, are less well understood. In this work, we investigated the effects of arginine-glycine-aspartate (RGD) ligand conformation (linear vs cyclic RGD) on primary human bone marrow stromal cell (hBMSC) and D1 stem cell osteogenic differentiation in three-dimensional (3D) culture and compared their response with that of committed MC3T3-E1 preosteoblasts to determine whether the stage of cell differentiation altered the response to the adhesion ligands. Linear RGD densities that promoted osteogenic differentiation of committed cells (MC3T3-E1 preosteoblasts) did not induce differentiation of hBMSCs or D1 stem cells, although matrices presenting the cyclic form of this adhesion ligand enhanced osteoprogenitor differentiation in 3D culture. This may be due to enhanced integrin-ligand binding. These studies indicate that biomaterial design parameters optimized for differentiated cell types may not directly translate to stem cell populations, because less-committed cells may require more instruction than differentiated cells. It is likely that design of synthetic extracellular matrices tailored to promote stem cell differentiation may enhance bone regeneration by transplanted cells. |
| Databáze: | OpenAIRE |
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