Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves
Autor: | K. Zlinszky, Ernst Peterhans, Christoph Egli, Monika Hilbe, Felix Ehrensperger, Hanspeter Stalder, Christian Schelp, Marlies Nussbaumer, Michael Haessig |
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Rok vydání: | 2007 |
Předmět: |
0301 basic medicine
Diagnostic methods Genotype 040301 veterinary sciences Enzyme-Linked Immunosorbent Assay Antibodies Viral Polymerase Chain Reaction Virus Serology 0403 veterinary science 03 medical and health sciences Antigen Animals Genotyping Skin Diarrhea Viruses Bovine Viral General Veterinary biology 04 agricultural and veterinary sciences Virology 030104 developmental biology Immunology biology.protein Immunohistochemistry Bovine Virus Diarrhea-Mucosal Disease Cattle Antibody |
Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 19(1) |
ISSN: | 1040-6387 |
Popis: | Five diagnostic techniques performed on skin biopsies (shoulder region) and/or serum were compared for detection of bovine viral diarrhea virus infection in 224 calves 0–3 months of age, 23 calves older than 3 months but younger than 7 months, and 11 cattle older than 7 months. The diagnostic methods used were immunohistochemistry (IHC), 2 commercial antigen ELISAs, 1 commercial antibody ELISA, and realtime RT-PCR. Results of 249 out of 258 skin and serum samples were identical and correlated within the 3 antigen detection methods and the real-time RT-PCR used. Twenty-six of these 249 samples were BVDV-positive with all antigen detection methods and the real-time RT-PCR. Nine out of 258 samples yielding discordant results were additionally examined by RT-PCR, RT-PCR Reamplification (ReA), and antigen ELISA I on serum and by immunohistochemistry on formalin fixed and paraffin-embedded skin biopsies. Virus isolation and genotyping was performed as well on these discordant samples. In 3 cases, transiently infected animals were identified. Two samples positive by real-time RT-PCR were interpreted as false positive and were ascribed to cross-contamination. The antigen ELISA II failed to detect 2 BVDV-positive calves due to the presence of maternal antibodies; the cause of 2 false-positive cases in this ELISA remained undetermined. Only persistently infected animals were identified in skin samples by IHC or antigen ELISA I. The 3 antigen detection methods and the real-time RT-PCR used in parallel had a high correlation rate (96.5%) and similar sensitivity and specificity values. |
Databáze: | OpenAIRE |
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