Effects of a novel synthetic retinoid on malignant glioma in vitro: inhibition of cell proliferation, induction of apoptosis and differentiation
| Autor: | E. Paillaud, J.L. Plassat, A Perzelova, C. Rochette-Egly, Marcienne Tardy, C. Fages, H Jouault, Silvia Lima Costa |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
medicine.drug_class Antineoplastic Agents Apoptosis Retinoids chemistry.chemical_compound Downregulation and upregulation Glutamine synthetase Tumor Cells Cultured medicine Humans Retinoid Glial fibrillary acidic protein biology Cell growth Glioma Immunohistochemistry Cell biology Cell Transformation Neoplastic Oncology chemistry Biochemistry Cell culture biology.protein Growth inhibition |
| Zdroj: | European Journal of Cancer. 37:520-530 |
| ISSN: | 0959-8049 |
| DOI: | 10.1016/s0959-8049(00)00430-5 |
| Popis: | Among six synthetic retinoids tested, the retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (CD437) was highly efficient in inducing growth inhibition of 8MG-BA and GL-15 human glioblastoma cell lines, with growth arrest at the S phase of the cell cycle. CD 437 also induced apoptosis in these cells, with 8MG-BA being the most sensitive. In these cells, induction of apoptosis by CD437 has been related to the downregulation of Bcl-2 expression and to CPP32 activation, but not to p53 expression. The remaining non-apoptotic cells presented a morphological pattern of astroglial differentiation with overexpression of glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS). The mechanism of action of CD437, originally developed as a RARgamma agonist, is not yet elucidated. However, our results suggest that it acts through an increase of the expression of retinoid-inducible genes, such as RARbeta2 and/or RARalpha2. |
| Databáze: | OpenAIRE |
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