Combination of horseradish peroxidase and lucifer yellow staining for selective labeling of neurons at the electron microscopic level
| Autor: | F Nagy, M Moulins, L Nonnotte, Alain Buisson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Nervous system
Histology Tissue Fixation Horseradish peroxidase law.invention chemistry.chemical_compound law Stomatogastric nervous system medicine Animals Horseradish Peroxidase Fluorescent Dyes Neurons Lucifer yellow biology Isoquinolines Staining Nephropidae Microscopy Electron medicine.anatomical_structure chemistry Biochemistry biology.protein Neuron Anatomy Electron microscope Neuroanatomy |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 39(11) |
| ISSN: | 0022-1554 |
| Popis: | We have developed a new double labeling method for electron microscopy to characterize selectively two physiologically identified neurons on the same preparation. The stomatogastric nervous system of crustaceans was used to test the distinguishing staining characteristics of the two labels. Neurons were labeled on one side with horseradish peroxidase (HRP) and on the other side with Lucifer yellow (LY). After blue light irradiation of the tissue in the presence of diaminobendizine, the two labeled neurons could be easily observed and discriminated on the same section by the two different reaction products. This simple technique of double labeling is useful in experimental neuroanatomy for the detailed study of synaptic relationships. |
| Databáze: | OpenAIRE |
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