Helicobacter pylori-specific CD4+ T-cell clones from peripheral blood and gastric biopsies
| Autor: | Sergio Abrignani, Rino Rappuoli, M T De Magistris, Piero Pileri, A Di Tommaso, Z Xiang, Natale Figura, M Bugnoli, P. F. Bayeli |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Biopsy Immunology Receptors Antigen T-Cell Chronic gastritis Immunoglobulins Major histocompatibility complex Microbiology Peripheral blood mononuclear cell Immune system Antigen HLA Antigens Gastric mucosa medicine Cytotoxic T cell Humans Antibody-Producing Cells B-Lymphocytes biology Helicobacter pylori T-Lymphocytes Helper-Inducer Middle Aged medicine.disease biology.organism_classification Clone Cells Infectious Diseases medicine.anatomical_structure Gastric Mucosa biology.protein Parasitology Female Research Article |
| Zdroj: | Europe PubMed Central ResearcherID |
| Popis: | Colonization of human gastric mucosa with cytotoxic strains of the bacterium Helicobacter pylori is associated with peptic ulcer and with chronic gastritis. Since little is known about the T-cell response to H. pylori, we investigated the CD4+ T-cell response both in peripheral blood mononuclear cells (PBMCs) and at the site of infection. First, we compared the bulk PBMC proliferative response to the bacterium in individuals with and without symptoms of gastroduodenal disease. We found that the PBMCs from virtually all individuals proliferate in response to heat-inactivated bacteria. Second, we cloned H. pylori-specific CD4+ T lymphocytes from the PBMCs of three patients and from both the gastric mucosa and PBMCs of a fourth patient. We have found that CD4+ T-cell clones specific for H. pylori from peripheral blood samples and gastric mucosae of infected patients are major histocompatibility complex class II restricted and discriminate between several cytotoxic and noncytotoxic bacterial strains. Moreover, they are polyclonal in terms of T-cell receptor usage and major histocompatibility complex restriction. Our results demonstrate that the T-cell response to the whole bacterium in PBMCs does not correlate with antibody response, infection, or disease. However, H. pylori-specific CD4+ T cells are detectable, at the clonal level, in both the periphery and gastric mucosa of infected patients. Localization of these cells at the site of disease suggests they are effectors of the immune response to the bacteria. |
| Databáze: | OpenAIRE |
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