Cyclical changes in sperm volume during in vitro incubation under capacitating conditions: a novel boar semen characteristic
| Autor: | R. Petzoldt, K. F. Weitze, R. A. P. Harrison, A. M. Petrounkina, Edda Töpfer-Petersen |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Osmosis Periodicity endocrine system Embryology BOAR Swine Semen Biology Andrology Endocrinology Capacitation Animals Acrosome Incubation Cells Cultured Cell Size Osmotic concentration urogenital system Obstetrics and Gynecology Cell Biology Spermatozoa Sperm Reproductive Medicine Immunology Sperm Capacitation Semen Preservation |
| Zdroj: | Reproduction. 118:283-293 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | The osmotic reactivity of boar spermatozoa during incubation in vitro was studied using a hypo-osmotic swelling test in conjunction with electronic measurement of cell volume. Sperm populations showed fluctuations in both iso-osmotic cell volume and hypo-osmotic volume response that fitted mathematical models for periodicity. Significant differences of frequency and amplitude were observed during sperm incubation under capacitating conditions as compared with those under non-capacitating conditions. In addition, different boars showed specific differences in their fluctuation characteristics under capacitating conditions. During incubation under capacitating conditions, a decrease in osmotic reactivity was observed that correlated with a decrease in motility, while the absolute value of the earliest maximum of the osmotic-induced response correlated with an increase in the proportion of discharged acrosomes. The time course of the cyclical behaviour of osmotic reactivity may be a useful parameter for assessing boar sperm response to capacitating conditions. |
| Databáze: | OpenAIRE |
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