Evidence of chondrocyte repopulation in adult ovine articular cartilage following cryoinjury and long-term transplantation
| Autor: | Kelli Novak, Ken Muldrew, Jerome B. Rattner, Locksley E. McGann, May Chung, Norman S. Schachar, John R. Matyas, R.F. Zernicke |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cartilage
Articular Cell Survival Type II collagen Biomedical Engineering Mitosis Osteoarthritis Matrix (biology) Biology Chondrocyte Cryopreservation Andrology Chondrocytes Rheumatology medicine Animals Transplantation Homologous Orthopedics and Sports Medicine RNA Messenger Sheep Regeneration (biology) Cartilage medicine.disease Transplantation Articular cartilage Chondrocyte Transplantation Osteoarthritis medicine.anatomical_structure Models Animal Immunology Female Collagen Cell Division |
| Zdroj: | Osteoarthritis and Cartilage. (5):432-439 |
| ISSN: | 1063-4584 |
| DOI: | 10.1053/joca.2000.0409 |
| Popis: | Objective To characterize the response of articular chondrocytes to a specific cryoinjury that leads to cluster formation following long-term transplantation. Design Osteochondral dowels from 20 adult sheep were cryopreserved to optimize the recovery of chondrocytes immediately after thawing. The dowels were transplanted as allografts and observed at 3 and 12 months. Chondrocyte distribution and viability was assessed using paravital dyes after transplantation. Chondrocyte phenotype was assessed by in situ hybridization and immunohistochemistry to detect type II collagen. An anticentrosome antibody was used to identify cells undergoing cell cycle progression towards mitosis. Results All cryopreserved grafts showed the presence of spheroidal clusters of chondrocytes 1 year after transplantation while the host cartilage adjacent to the graft appeared morphologically normal. The average size of the clusters increased from four cells at 3 months to 12 cells at 1 year. The chondrocytes in the clusters displayed newly formed type II collagen protein and mRNA. Some cells within clusters were observed with two centrosomes, indicative of cells progressing through the S phase of the cell cycle. Conclusion Adult articular chondrocytes retain the ability to repopulate the matrix, an ability which is demonstrated with this specific cryoinjury. This may be an initial stage of cartilage regeneration. |
| Databáze: | OpenAIRE |
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