The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area

Autor: Eloina Gonzalez Mancebo, K T B Pelgrom, R. van Ree, C.I. Matos, Elma M. J. Salentijn, Luud J.W.J. Gilissen, Laurian Zuidmeer, Montserrat Fernandez Rivas, R Asero
Přispěvatelé: Amsterdam institute for Infection and Immunity, Experimental Immunology, Amsterdam Public Health
Rok vydání: 2006
Předmět:
cross-reactivity
contact urticaria
fruits
Basophil
medicine.disease_cause
Immunoglobulin E
Histamine Release
Cross-reactivity
Cohort Studies
Profilins
chemistry.chemical_compound
Laboratorium voor Plantenveredeling
foods
PRI Biodiversiteit en Veredeling
antibodies
Immunology and Allergy
birch pollen
Plant Proteins
swiss-model
biology
Fragaria
Recombinant Proteins
Basophils
PRI Bioscience
bet-v-1
medicine.anatomical_structure
Italy
Profilin
Plant lipid transfer proteins
Food Hypersensitivity
Histamine
vegetables
Immunoblotting
Immunology
Cross Reactions
PRI BIOS Applied Genomics & Proteomics
Microbiology
Radioallergosorbent Test
Double-Blind Method
medicine
Humans
cDNA library
Allergens
Antigens
Plant
PRI Biodiversity and Breeding
Plant Breeding
chemistry
Spain
biology.protein
identification
Carrier Proteins
Zdroj: Clinical and experimental allergy, 36(5), 666-675. Wiley-Blackwell
Clinical and Experimental Allergy 36 (2006) 5
Clinical and Experimental Allergy, 36(5), 666-675
ISSN: 1365-2222
0954-7894
Popis: Summary Background In contrast to other Rosaceae fruit, only few cases of patients with adverse reactions to strawberry are listed in literature. Objective To identify allergenic proteins in strawberry and to express and characterize recombinant strawberry lipid transfer protein (LTP; rFra a 3). Methods Established apple-allergic patients were recruited on the basis of a reported allergic reaction to strawberry (n=28, confirmed by double-blind placebo-controlled food challenge in four patients) or on the basis of IgE reactivity to LTP (n=34). Sensitization to purified natural and recombinant allergens was assessed by RAST, immunoblot (inhibition) and basophil histamine release (BHR). A strawberry cDNA library was screened for genes homologous to known fruit allergens. Fra a 3 was cloned and expressed in the yeast Pichia pastoris and compared with peach and apple LTP by RAST, immunoblot-inhibition and BHR tests. Results Genes homologous to Bet v 1, Bet v 6, profilin and LTP were identified in a strawberry cDNA library. In BHR the rFra a 3 induced histamine release at a 100-fold higher concentration than peach LTP. RAST inhibition showed high cross-reactivity to peach and apple LTP, although IgE reactivity was lower by a factor 5. On strawberry immunoblot, patients' IgE showed reactivity to a Bet v 1 homologue, profilin, LTP and high-molecular weight bands. Conclusion In addition to a Bet v 1 homologue, strawberry also contains IgE-binding profilin and LTP. The rFra a 3 has less allergenic potency than peach and apple LTP, and therefore is an interesting tool for future immunotherapy. Fra a 3 does not seem to be clinically relevant.
Databáze: OpenAIRE
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