Structural insights in interactions between RNase from Bacillus Intermedius and rhamnogalacturonan I from potato
| Autor: | Olga Makshakova, E.R. Safarova, Yu. F. Zuev |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Polymers and Plastics
RNase P Molecular Conformation Bacillus 02 engineering and technology 010402 general chemistry Polysaccharide 01 natural sciences chemistry.chemical_compound Ribonucleases Bacterial Proteins Materials Chemistry Side chain Ribonuclease Solanum tuberosum chemistry.chemical_classification biology Chemistry Organic Chemistry Galactan 021001 nanoscience & nanotechnology 0104 chemical sciences Polysaccharide binding Molecular Docking Simulation Biochemistry Docking (molecular) Drug delivery biology.protein Pectins 0210 nano-technology Protein Binding |
| Zdroj: | Carbohydrate polymers. 251 |
| ISSN: | 1879-1344 |
| Popis: | Being biocompatible and biodegradable polymers, polysaccharides present a perspective material for drug delivery systems. This study aimed at unraveling the molecular details of interactions between rhamnogalacturonan I, brunched with galactan side chains, and RNase from Bacillus Intermedius, binase. FTIR- and NMR-spectroscopic analyses showed that binase interacts with side chains of the polysaccharide. In complexes with polysaccharide, the protein retains its native structure. The 2D-NMR techniques revealed eight protein residues responsive to polysaccharide binding. Further, computer simulations were carried out to provide the atomistic details of binase-polysaccharide complexes. Both blind and knowledge-based docking procedures elucidate the existence of epitopes on the binase surface with the preferential binding of galactan fragments. The refinement of these complexes by molecular dynamics simulations confirmed stable protein-polysaccharide interactions. The results of this study strengthen the knowledge on non-specific protein-carbohydrate interactions and outline the rhamnogalacturonan I as a possible matrix material for protein delivery systems. |
| Databáze: | OpenAIRE |
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