Low Molar Mass Dextran: One-Step Synthesis With Dextransucrase by Site-Directed Mutagenesis and its Potential of Iron-Loading
| Autor: | Zhiming Jiang, Fang Yan, Jiangfeng Ma, Bin Wu, Tingting Wang, Min Jiang, Weiliang Dong, Hao Wu, Yiya Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Histology
Molar mass Stereochemistry dextransucrase low molar mass dextran Dispersity Biomedical Engineering Bioengineering and Biotechnology Substrate (chemistry) Bioengineering Dextransucrase Homopolysaccharide chemistry.chemical_compound Dextran chemistry iron dextran GH70 family medicine Molar mass distribution Ferric site-directed mutagenesis TP248.13-248.65 Original Research Biotechnology medicine.drug |
| Zdroj: | Frontiers in Bioengineering and Biotechnology, Vol 9 (2021) Frontiers in Bioengineering and Biotechnology |
| ISSN: | 2296-4185 |
| DOI: | 10.3389/fbioe.2021.747602 |
| Popis: | Iron dextran is a common anti-anemia drug, and it requires low molar mass dextran as substrate. In this work, we selected 11 amino acid residues in domain A/B of DSR-MΔ2 within a 5-angstrom distance from sucrose for site-directed mutagenesis by molecular docking. Mutation of Q634 did not affect the enzyme catalytic activity, but showed an obvious impact on the ratio of low molecular weight dextran (L-dextran, 3,000–5,000 Da) and relatively higher molecular weight dextran (H-dextran, around 10,000 Da). L-dextran was the main product synthesized by DSR-MΔ2 Q634A, and its average molecular weight was 3,951 Da with a polydispersity index |
| Databáze: | OpenAIRE |
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