Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2
| Autor: | Chantal Reusken, Adam Meijer, Annemiek A. van der Eijk, Kamelia R. Stanoeva, Marion Koopmans, L. M. Kortbeek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Coronavirus disease 2019 (COVID-19) Isolation (health care) Epidemiology Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology Laboratory Diagnosis Review Sensitivity and Specificity Polymerase Chain Reaction 03 medical and health sciences COVID-19 Testing 0302 clinical medicine SDG 3 - Good Health and Well-being Virology Humans Medicine 030212 general & internal medicine Intensive care medicine SARS-CoV-2 business.industry Public Health Environmental and Occupational Health COVID-19 Timeline Molecular diagnostics Molecular Diagnostic Techniques Good clinical practice Outbreak control business Contact tracing |
| Zdroj: | Eurosurveillance |
| ISSN: | 1025-496X |
| Popis: | BackgroundSensitive molecular diagnostics and correct test interpretation are crucial for accurate COVID-19 diagnosis and thereby essential for good clinical practice. Furthermore, they are a key factor in outbreak control where active case finding in combination with isolation and contact tracing are crucial.AimWith the objective to inform the public health and laboratory responses to the pandemic, we reviewed current published knowledge on the kinetics of SARS-CoV-2 infection as assessed by RNA molecular detection in a wide range of clinical samples.MethodsWe performed an extensive search on studies published between 1 December 2019 and 15 May 2020, reporting on molecular detection and/or isolation of SARS-CoV-2 in any human laboratory specimen.ResultsWe compiled a dataset of 264 studies including 32,515 COVID-19 cases, and additionally aggregated data points (n = 2,777) from sampling of 217 adults with known infection timeline. We summarised data on SARS-CoV-2 detection in the respiratory and gastrointestinal tract, blood, oral fluid, tears, cerebrospinal fluid, peritoneal fluid, semen, vaginal fluid; where provided, we also summarised specific observations on SARS-CoV-2 detection in pregnancy, infancy, children, adolescents and immunocompromised individuals.ConclusionOptimal SARS-CoV-2 molecular testing relies on choosing the most appropriate sample type, collected with adequate sampling technique, and with the infection timeline in mind. We outlined knowledge gaps and directions for future well-documented systematic studies. |
| Databáze: | OpenAIRE |
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