Generation of natural killer cells from serum-free, expanded human umbilical cord blood CD34+ cells
| Autor: | Chao-Ling Yao, Mei-Ling Wu, I-Ting Kao, Tzu-Lin Chuang, Shiaw-Min Hwang, Zwe-Ling Kong |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Cell Survival
Antigens CD34 Umbilical Cord Interleukin 21 Pregnancy Humans Antigen-presenting cell Lymphokine-activated killer cell CD40 biology Janus kinase 3 Cell Differentiation Cell Biology Hematology Natural killer T cell Fetal Blood Hematopoietic Stem Cells Molecular biology Culture Media Killer Cells Natural Immunology Myeloid-derived Suppressor Cell biology.protein Interleukin 12 Female Developmental Biology |
| Zdroj: | Stem cells and development. 16(6) |
| ISSN: | 1547-3287 |
| Popis: | Natural killer (NK) cells are important effectors of the innate immune system, which exhibits cytolytic activity against infectious agents and tumor cells. NK cells are derived from CD34(+) hematopoietic stem cells (HSCs). Human umbilical cord blood (UCB) has been recognized as a rich source of HSCs. Previously, we have reported an optimized serum-free medium for ex vivo expansion of CD34(+) cells from UCB. In this study, the serum-free, expanded CD34(+) cells were tested to differentiate into NK cells and their induction kinetics. After 5 weeks of induction, the induced NK cells were characterized by analysis of surface antigens, IFN-gamma secretion, and cytotoxicity against K562 cells. The results indicated that NK cells derived from the serum-free, expanded CD34(+) cells exhibited both characteristics and functions of NK cells. Furthermore, the serum-free, expanded CD34(+) cells showed a significantly higher NK cell differentiation potential than freshly isolated CD34(+) cells. NK cells induced from serum-free, expanded CD34(+) cells showed a higher concentration of IFN-gamma secretion and ability of cytotoxicity than those from freshly isolated CD34(+) cells. Therefore, ex vivo-expanded CD34(+) cells in optimized serum-free medium could differentiate into NK cells and provided a promising cell source for immunotherapeutic approaches. |
| Databáze: | OpenAIRE |
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