Visualization of dendritic cells' responses in atopic dermatitis: Preventing effect of emollient
| Autor: | Jimmy Le Digabel, Jérôme Filiol, Etienne Gontier, Daniel Redoules, Gwendal Josse, Céline Mias, Eléonore Gravier, Emmanuel Questel, Thérèse Nocera, Ana-Beatris Rossi, Aurélie Villaret |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Skin barrier medicine.medical_specialty Dermatophagoides pteronyssinus Dermatology Biochemistry Dermatitis Atopic Atopy 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Molecular Biology integumentary system Epidermis (botany) Emollients Chemistry Inflammatory skin disease Patch test Atopic dermatitis Patch Tests medicine.disease Suction blister 030104 developmental biology Microscopy Fluorescence Multiphoton 030220 oncology & carcinogenesis Langerhans Cells Immunology Epidermis Infiltration (medical) |
| Zdroj: | Experimental dermatology. 27(4) |
| ISSN: | 1600-0625 |
| Popis: | Atopic dermatitis (AD) is a chronic and multifactorial inflammatory skin disease involving various dendritic cells such as epidermal Langerhans cells (LC) and inflammatory dendritic epidermal cells (IDECs). Most of the clinical studies was performed on isolated cells, and thus, it would be useful to characterize directly on the human epidermal tissue the first cellular events occurred during the AD. The suction blister method was used to obtain whole epidermis samples and interstitial cutaneous fluids. Employing multiphoton microscopy, we analyzed the early dynamic behavior of inflammatory cells using Dermatophagoides pteronyssinus atopy patch test (Derp-APT) and evaluated the effects of emollient pre-application. Derp-APT application provoked rapid and strong infiltration of IDECs, and proliferation and activation of LC in the AD subjects' epidermis. Moreover, emollient pre-application strengthened the defective skin barrier and had positive effects on inflammatory cells' behavior, characterized by the complete inhibition of IDEC influx and the presence of immature LC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text