Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment
| Autor: | M. E. J. De Jonge, E. C. Wolters, G. T. Noordhoek, J. D. A. Van Embden |
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| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Microbiology (medical) medicine.drug_class Molecular Sequence Data Antibiotics Biology urologic and male genital diseases Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Neurosyphilis Cerebrospinal fluid law medicine Humans Treponema pallidum Polymerase chain reaction Treponema Base Sequence medicine.disease biology.organism_classification Virology Penicillin Oligodeoxyribonucleotides Evaluation Studies as Topic Penicillin G Benzathine Syphilis Primer (molecular biology) Research Article medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 29:1976-1984 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.29.9.1976-1984.1991 |
| Popis: | A polymerase chain reaction with nested primer pairs based on the DNA sequence of the 39-kDa bmp gene of Treponema pallidum subsp. pallidum is described. The method allowed the detection of purified T. pallidum DNA equivalent to the amount of DNA in a single bacterium and was specific for T. pallidum subspecies. After concentration of DNA, using diatomaceous earth, it was possible to detect about 100 treponemes in 1 ml of cerebrospinal fluid. Cerebrospinal fluid samples from a total of 29 symptomatic and asymptomatic patients with neurosyphilis were tested for the presence of treponemal DNA before and at various intervals after intravenous treatment with penicillin. Prior to the penicillin treatment, we detected T. pallidum DNA in 5 of 7 patients with acute symptomatic neurosyphilis, in none of the 4 patients with chronic symptomatic neurosyphilis tested before treatment, and in 2 of 16 patients with asymptomatic neurosyphilis. Unexpectedly, T. pallidum DNA was also often detected in cerebrospinal fluid long after intervenous treatment with penicillin, sometimes up to 3 years after therapy. |
| Databáze: | OpenAIRE |
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