Direct binding of the Mtv7 superantigen (Mls-1) to soluble MHC class II molecules
| Autor: | David G. Mottershead, Robert G. Urban, Ping-Ning Hsu, Brigitte T. Huber, Jack L. Strominger |
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| Rok vydání: | 1995 |
| Předmět: |
CD74
Molecular Sequence Data Immunology chemical and pharmacologic phenomena law.invention Minor Lymphocyte Stimulatory Antigens 03 medical and health sciences Mice 0302 clinical medicine law MHC class I Superantigen Escherichia coli Animals Humans Immunology and Allergy Antigens Viral 030304 developmental biology 0303 health sciences MHC class II Binding Sites biology Base Sequence Mouse mammary tumor virus Histocompatibility Antigens Class II MHC restriction biology.organism_classification Molecular biology In vitro Recombinant Proteins Infectious Diseases biology.protein Recombinant DNA 030215 immunology Plasmids |
| Zdroj: | Immunity. 2(2):149-154 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/s1074-7613(95)80027-1 |
| Popis: | Summary The superantigen encoded by the mouse mammary tumor virus (MMTV) is a potent stimulator of T cells when bound to MHC class II molecules. Recent data from this laboratory have shown that the Mtv7 superantigen, Mls-1, elicits a strong T cell response when presented by HLA-DR. To expand these observations further, we have produced the 28 kDa extracellular domain and the 18 kDa carboxy-terminal subfragment of the Mls-1 protein in E. coli and studied their interaction with human MHC class II molecules in vitro. In this report, we demonstrate direct binding of these recombinant forms of the Mls-1 protein to soluble HLA-DR1 and HLA-DR4, but not to HLA-A2. Our data imply a unique class II interaction site of retroviral superantigens that is not shared with bacterial superantigens. |
| Databáze: | OpenAIRE |
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