Semen cassiae Extract Inhibits Contraction of Airway Smooth Muscle
| Autor: | Shanshan Chen, Qian Wang, Wen-Jing Zhang, Yong-Bo Peng, Jingyu Chen, Xiao-Xue Zhao, Hao Xu, Lei Cao, Weiwei Chen, Jinhua Shen, Chenyou Shen, Meng-Fei Yu, Shu Chen, Meng-Yue Li, Qing-Hua Liu, Bei-Bei Liu, Dun-An Zang, Xiaowei Nie, Gangjian Qin, Xi Luo, Ping Zhao, Yu-Shan She, Jun-Ying Qiu, Li-Qun Ma, Jiapei Dai, Yuan-Yuan Chen, Lu Xue |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Contraction (grammar) aurantio-obtusin Semen Pharmacology 03 medical and health sciences 0302 clinical medicine TRPC3 relaxation Cassia medicine Pharmacology (medical) Ion channel Original Research COPD Lung biology Chemistry lcsh:RM1-950 contraction ion channels medicine.disease biology.organism_classification airway smooth muscle 030104 developmental biology medicine.anatomical_structure lcsh:Therapeutics. Pharmacology 030220 oncology & carcinogenesis Semen cassiae Intracellular |
| Zdroj: | Frontiers in Pharmacology, Vol 9 (2018) Frontiers in Pharmacology |
| ISSN: | 1663-9812 |
| DOI: | 10.3389/fphar.2018.01389/full |
| Popis: | β2-adrenoceptor agonists are commonly used as bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease (COPD), however, they induce severe side effects. Therefore, developing new bronchodilators is essential. Herbal plants were extracted and the extracts' effect on airway smooth muscle (ASM) precontraction was assessed. The ethyl alcohol extract of semen cassiae (EESC) was extracted from Semen cassia. The effects of EESC on the ACh- and 80 mM K+-induced sustained precontraction in mouse and human ASM were evaluated. Ca2+ permeant ion channel currents and intracellular Ca2+ concentration were measured. HPLC analysis was employed to determine which compound was responsible for the EESC-induced relaxation. The EESC reversibly inhibited the ACh- and 80 mM K+-induced precontraction. The sustained precontraction depends on Ca2+ influx, and it was mediated by voltage-dependent L-type Ca2+ channels (LVDCCs), store-operated channels (SOCs), TRPC3/STIM/Orai channels. These channels were inhibited by aurantio-obtusin, one component of EESC. When aurantio-obtusin removed, EESC's action disappeared. In addition, aurantio-obtusin inhibited the precontraction of mouse and human ASM and intracellular Ca2+ increases. These results indicate that Semen cassia-contained aurantio-obtusin inhibits sustained precontraction of ASM via inhibiting Ca2+-permeant ion channels, thereby, which could be used to develop new bronchodilators. |
| Databáze: | OpenAIRE |
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