Overexpression of microRNA-1288 in oesophageal squamous cell carcinoma

Autor: Simon Law, Johnny Cheuk On Tang, Vinod Gopalan, Kwok Wah Chan, Suja Pillai, Daniel King Hung Tong, Alfred King-Yin Lam, Farhadul Islam
Rok vydání: 2016
Předmět:
Adult
Cell Extracts
Male
0301 basic medicine
Esophageal Neoplasms
Down-Regulation
Fluorescent Antibody Technique
FOXO1
Biology
Transfection
Immunofluorescence
medicine.disease_cause
Basement Membrane
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Western blot
Cell Line
Tumor
microRNA
medicine
Humans
neoplasms
Tumor Stem Cell Assay
Aged
Cell Proliferation
Aged
80 and over
medicine.diagnostic_test
Forkhead Box Protein O1
Cell growth
Reproducibility of Results
Cell migration
Cell Biology
Middle Aged
Survival Analysis
digestive system diseases
Clone Cells
Gene Expression Regulation
Neoplastic
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Cancer research
Female
Esophageal Squamous Cell Carcinoma
KRAS
Neoplasm Grading
Zdroj: Experimental Cell Research. 348:146-154
ISSN: 0014-4827
Popis: Purpose This study aims to examine the expression profiles miR-1288 in oesophageal squamous cell carcinoma (ESCC). The cellular implications and target interactions of ESCC cells following miR-1288 overexpression was also examined. Methods In total, 120 oesophageal tissues (90 primary ESCCs and 30 non-neoplastic tissues) were recruited for miR-1288 expression analysis using qRT-PCR. An exogenous miR-1288 mimic and its inhibitor were used to explore the in-vitro effects of miR-1288 on ESCC cells by performing cell proliferation, colony formation, cell invasion and migration assays. Localisation and modulatory changes of various miR-1288 regulated proteins such as FOXO1, p53, TAB3, BCL2 and kRAS was examined using immunofluorescence and western blot. Results Overexpression of miR-1288 was more often noted in ESCC tissues when compared to non-neoplastic oesophageal tissues. High expression was often noted in high grade carcinomas and with metastases. Patients with high levels of miR-1288 expression showed a slightly better survival compared to patients with low miR-1288 levels. Furthermore, overexpression of miR-1288 showed increased cell proliferation and colony formation, improved cell migration and enhanced cell invasion properties in ESCC cells. In addition, miR-1288 overexpression in ESCC cells showed repression of cytoplasmic tumour suppressor FOXO1 protein expression. Inversely, inhibition of miR-1288 expression exhibited remarkable upregulation of FOXO1 protein, while expressions of other tested proteins remain unchanged. Conclusions Up regulation of miR-1288 expression in ESCC tissues and miR-1288 induced oncogenic features of ESCC cells in-vitro indicates the oncogenic roles of miR-1288 in ESCCs. Overexpression of miR-1288 play a key role in the pathogenesis of ESCCs and its modulation may have potential therapeutic value in patients with ESCC.
Databáze: OpenAIRE
Externí odkaz: