Isolation of a cytotoxin from L-form Salmonella typhimurium
| Autor: | Akira Nakano, Noriaki Kamikaidou, Shuzo Kashiba, Eiji Kita |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Salmonella typhimurium
Salmonella Lysis Bacterial Toxins Biology medicine.disease_cause Microbiology Mice In vivo Genetics medicine Animals Thermolabile Molecular Biology Mice Inbred C3H Molecular mass Toxin Isoelectric focusing Cytotoxins Tumor Necrosis Factor-alpha Macrophages Chromatography Ion Exchange Endotoxins Isoelectric point Chromatography Gel Isoelectric Focusing |
| Zdroj: | FEMS microbiology letters. 109(2-3) |
| ISSN: | 0378-1097 |
| Popis: | A cytotoxic protein was isolated from the sodium dodecyl sulphate (SDS)-solubilized extract of the stable L forms of Salmonella typhimurium by ion-retardation chromatography, ion-exchange chromatography, isoelectric focusing and gel filtration. The purified toxin, with a molecular mass of 32 kDa and with isoelectric point of 6.4, was thermolabile and trypsin-sensitive. Against mouse macrophages, its cytolytic effect was detectable in vitro at concentrations higher than 0.7 micrograms/ml, with a complete lysis obtained at 5 micrograms/ml. In contrast, it stimulated C3H/HeJ macrophages in the dose range of 0.1-0.5 micrograms/ml to allow the cell to respond to endotoxin, resulting in the significant production of tumor necrosis factor alpha. By Northern blot analysis, this effect was detectable at a dose as low as 0.01 micrograms/ml. These findings suggest that the transformation of bacillary S. typhimurium into L forms in vivo may induce alterations in host resistance against murine typhoid. |
| Databáze: | OpenAIRE |
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