Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin
| Autor: | Mateus Matiuzzi da Costa, Sanely Lourenço da Costa, Mariana de Barros, Richard Costa Polveiro, Maria Aparecida Scatamburlo Moreira, Laís Karolyne de Castro, Thalita Moreira Scatamburlo, Magna Coroa Lima, Samuel Henrique Sales Guimarães |
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| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Virulence Factors lcsh:QR1-502 Virulence Mastitis Microbial Sensitivity Tests Biology medicine.disease_cause Caprine mastitis Microbiology lcsh:Microbiology Persistence 03 medical and health sciences Bacterial Proteins Drug Resistance Bacterial medicine Enrofloxacin Animals Phylogeny 030304 developmental biology 0303 health sciences Goat Diseases 030306 microbiology Goats Gene Expression Regulation Bacterial Staphylococcal Infections medicine.disease Antimicrobial biology.organism_classification Anti-Bacterial Agents Small ruminants Parasitology Female Efflux Bacteria Multilocus Sequence Typing Research Article medicine.drug |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 20, Iss 1, Pp 1-11 (2020) |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-020-01793-9 |
| Popis: | Background Staphylococcus aureus is one of the main causative agents of mastitis in small ruminants. Antimicrobial use is the major treatment, but there are many flaws linked to resistance, tolerance or persistence. This study aimed to verify changes in resistance, virulence and clonal profiles of S. aureus isolated from persistent mastitis goat milk before and after enrofloxacin treatment. Results MIC increased to at least one antimicrobial in S. aureus isolates after enrofloxacin treatment compared to before. The most detected resistance genes before and after treatment were tetK, tetM, and blaZ, with more resistance genes detected after enrofloxacin treatment (p hla, fnbA, fnbB, eta, etb, sea, sec, seh, and sej) were detected at both times, and between these, the hla and eta genes were detected more in isolates after treatment. All isolates of S. aureus belonged to the same sequence type (ST) 133, except for two S. aureus isolates prior to enrofloxacin treatment which were classified as ST5 and the other as a new one, ST4966. Isolates of S. aureus 4, 8, and 100 from before and after treatment had identical pulse types, while others obtained from other animals before and after treatment were classified into distinct pulse types. Conclusion There were occasional changes in the studied profiles of S. aureus isolated before and after treatment of animals with enrofloxacin, which may have contributed to the permanence of bacteria in the mammary gland, even when using traditional treatment, resulting in persistent mastitis. |
| Databáze: | OpenAIRE |
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